J Korean Pain Soc.  2002 Jun;15(1):13-18.

Effect of NMDA Receptor Antagonist and NOS Inhibitor on Spinal Sensory Neurons of Neuropathic Pain Animal Model

• Lee KC
• Choi YS
• Lee HB
• Park ST

Abstract

BACKGROUND: In order to evaluate the effect of N-methyl-D-aspartate (NMDA) receptor antagonist, D-2-amino-5-phosphonovaleric acid (APV), nitric oxide synthase (NOS) inhibitor, and aminoguanidine (AGH) on rat spinal sensory neurons from the neuropathic pain animal model.
METHODS
Cell viability, amount of neurofilament by immunocytochemistry, and rate of protein synthesis were measured after spinal motor neurons of rats were incubated with various concentrations of APV or AGH for 48 hours.
RESULTS
Cell viability of spinal sensory neurons from the neuropathic pain model was remarkably decreased compared with the control. Regarding their protective effect, both APV and AGH significantly increased cell viability, amount of neurofilament and rate of protein synthesis, compared to experimental groups with untreated APV or AGH in spinal sensory neurons.
CONCLUSIONS
The present study suggests that NMDA receptor, reactive nitrogen species (RNS), and NO are involved in neuropathic pain.

Keyword

NMDA receptor; Neuropathic pain animal model; Reactive nitrogen species; Spinal sensory neuron

MeSH Terms

Animals*
Cell Survival
Immunohistochemistry
Models, Animal*
Motor Neurons
N-Methylaspartate*
Neuralgia*
Nitric Oxide Synthase
Rats
Reactive Nitrogen Species
Sensory Receptor Cells*
N-Methylaspartate
Nitric Oxide Synthase
Reactive Nitrogen Species