Korean J Anesthesiol.  2001 Aug;41(2):222-228. 10.4097/kjae.2001.41.2.222.

The Effects of Nonspecific Cation Blocker Gadolinium on Hypoxic Pulmonary Vasoconstriction in the Isolated Rabbit Lung

• Kim SD
• Kim CS
• Kim HS
• Ahn WS
• Oh AY
• Cheong IY

• KMID: 1936189
• DOI: http://doi.org/10.4097/kjae.2001.41.2.222

Abstract

BACKGROUND
Hypoxic pulmonary vasoconstriction (HPV) is unique to pulmonary circulation. Recent hypotheses have emerged indicating that O2 levels per se can regulate ion channel activity. The modulation of both cation channels differs according to the conduit or resistance pulmonary vessel type. However, it is not yet studied that the cation channel blocker has the same effect in an animal experimental model, which can exclude several factors that may influence on HPV. The purpose of the present study was, therefore, to determine the effect of nonspecific cation blocker, Gadolinium, on HPV in a rabbit model of isolated lung perfusion.
METHODS
In adult white rabbits (n = 6), lungs were isolated and perfused with the constant pulmonary perfusate flow. Acid-base status and electrolytes of perfusate also constantly maintained. Thirty minutes after, baseline hypoxic pulmonary vasoconstriction (HPV) was measured as the difference of pulmonary artery pressure between a period of 21% normoxic gas inhalation and that of 3% hypoxic gas inhalation. After another thirty minutes, Gadolinium 50microgram were mixed to the perfusate, and then HPV were measured three times. After then Gadolinium 100, 200, 400microgram were mixed to the perfusate and HPV were measured.
RESULTS
Gadolinium decreased the HPV response according to the dose. The ED50 of the response was 143microgram/100 ml.
CONCLUSIONS
The regulation of HPV is based on the cation channel in the isolated rabbit lung.

Keyword

rabbit; Antagonist; cation channel bloker; gadolinium; hypoxic pulmonary vasoconstriction

MeSH Terms

Adult
Animal Experimentation
Electrolytes
Gadolinium*
Humans
Inhalation
Ion Channels
Lung*
Perfusion
Pulmonary Artery
Pulmonary Circulation
Rabbits
Vasoconstriction*
Electrolytes
Gadolinium
Ion Channels