Korean J Blood Transfus.  1995 Dec;6(2):155-160.

Evaluation of the Status of Frozen Thawed Platelet Concentrations By using 6% Dimethyl Sulfoxide Cryopreservation method

• Kim JS
• Kim BS
• Lee KN
• Lim CS
• Kim YK

Abstract

We tried to analyze the status of 10 units of frozen thawed apheresis platelet concentration by 6% DMSO method for the evaluation of practical applicability. The platelet concentrations were transferred to PL-732(Baxter, USA) cryopreservation bag, and DMSO is added to those bag at slow rate until expected final 6% concentration is achieved, thereafter those were directly placed to -80 degrees C refrigerator for freezing. Someday later from I week to 1 month, those were thawed at 37 degrees C water bath, and then washed by same volume of ABO matched plasma. In the course of cryopreservation, about 7% of platelets were lost and the mean recovery rate of platelet was 93% compared with those of unfrozen status. LDH, the values of platelet lysis, and pH were within normal limits, whereas platelet aggregation test shows decreased aggregation to collagen and ristocetine compared with those of unfrozen status(p<0.05) but they were clinically acceptable. We suggest that the frozen platelets may be useful in a some clinical situation such as hematologic malignancy and solid tumor by autologous transfusion.

MeSH Terms

Baths
Blood Component Removal
Blood Platelets*
Collagen
Cryopreservation*
Dimethyl Sulfoxide*
Freezing
Hematologic Neoplasms
Hydrogen-Ion Concentration
Plasma
Platelet Aggregation
Ristocetin
Water
Collagen
Dimethyl Sulfoxide
Ristocetin
Water