Korean J Blood Transfus.  1999 Dec;10(2):179-186.

Erythrocyte Depletion Effect of Compression Method with 6% Hydroxyethyl Starch in Human Umbilical Cord Blood

• Jung JT
• Chae YS
• Park SW
• Baik JH
• Kim DH
• Park SH
• Sohn SK
• Suh JS
• Lee J
• Lee KB

Abstract

BACKGROUND
Stem cell transplantation with high dose chemoradiotherapy has made it possible to cure a significant proportion of patients with hematopoietic malignancies. RBC purging is an essential step prior to transplantation in ABO mismatched setting. The purpose of this study is to develop a new simple method for RBC depletion from harvested stem cells.
METHODS
Ten cord bloods were collected during deliveries at Kyungpook National University Hospital, Taegu, Korea at August, 1998. All nucleated cells were collected after the compression of bags with 6% hydroxyethyl starch and cord blood for 4 hours. The cell viability of mononucleated cell (MNC) was calculated after 24~72 hours. RESLUTS: The collected mean volume of cord blood was 37.1 +/- 6.7 ml. The WBC count was 10,852 +/- 1,137/microL. The 3.90 +/- 0.62x108 TNC and 1.82 +/- 0.36x108 MNC were obtained per collection. TNC efficiency was 83.8 +/- 7.01% and MNC efficiency was 90.9 +/- 9.23% by compression method with 6% hydroxyethyl starch. RBC contamination was negligible. The cell viabilities of mononuclear cell were kept in a range of 99~100% after 24~72 hours.
CONCLUSION
Because compression method with 6% hydroxyethyl starch progresses in closed system is simple & easy, has high erythrocyte depletion efficacy, and can maintain high viability of stem cells, it can be used for RBC purging in ABO mismatched stem cell transplantation.

Keyword

Umbilical cord blood; Hydroxyethyl starch; Mononuclear cell; RBC purging

MeSH Terms

Cell Survival
Chemoradiotherapy
Daegu
Erythrocytes*
Fetal Blood*
Gyeongsangbuk-do
Hematologic Neoplasms
Humans*
Korea
Starch*
Stem Cell Transplantation
Stem Cells
Umbilical Cord*
Starch