Korean J Dermatol.
1968 Jul;6(1):19-24.
Experimental Study on Sporotrichum by Inoculation in Rats
Abstract
- Sporotrichsis is a chronic granulomatous mycosis caused by Sporotrichum species. In Korea, Kim et al. had reported two cases of cutaneous sporotrichosis involving upper extremities for the first time in 1960. They had determined their strain to be similar to a variant of S.beuermanni by culture. These Kim's strain has inoculated into the peritoneum of rats pathogenicity, virulence and course by examining the macroscopic The conclusion were as follows: 1. Rats were inoculated intraperitoneally and killed at intervals of 12, 20, 30, 40 and 50 days and followed by postmortem examination. Macroscopic findings: At twelve day and lesions were observed. At 20 to 50 days all showed grayish brown lymphnodules varying in size, which were disseminated on the mesentery, omentum, peritoneum and inguinal region. On lung, liver, spleen and kidney, various changes i. e.; congestion, necrosis and degenerative changes were noted at 30 to 40 days after inoculation. 3. Microscopic findings: a. Most nodules were characterized by three distinct zones, i. e.; central suppurative, mirldle tuberculoid, and peripheral syphiloid zones. b. Visceral organs showed the following pathological changes varying with the duration of infection; diffuse hepatic necrosis, bronchitis and pneumonitis, splenitis and degene- rative changes in the kidney. c. Microorganisms could found in tissue by PAS stain, Its frequency rate shows 52% in lymphnodules and 16% in visceral organs. 4. For some unknown reasons isolation of the organisms by culture was successful only from 3 rats.
