J Korean Ophthalmol Soc.  1967 Jun;8(1):45-49.

An Experimental Study on the Animal Intraocular Inoculation of toxoplasma gondii

• Kim HC

Abstract

Toxoplamosis has a world-wide distribution in humans, and nearly all domestic and wild animals may become affected. Toxoplasma is a protozoan parasite which produces either congenital or acquired chorioretinits. Most intraocular toxoplasmic chorioretinitis participates a granulomatous type of inflammation. Toxoplasmic uveitis are well-established entities. The exact method of transmission of the disease is not known. Since human ocular tissue cannot be subjected to a direct histologic study, such a diagnosis is always presumptive. This study on the animal inoculation of toxoplasma gondii, the strain of toxoplasma gondii has been shared from Veterinary Research Lab., Anyang, Korea on April, 1966. The experiment is planned in two ways. 1) One million living organisms were inoculated into subretinal space. 2) One fourth million living organisms were inoculated into anterior chamber. Thereafter the author observed the clinical signs with loupe, slit-lamp, and ophthalmoscope. One week later, animal inoculation of toxoplasma strain, the rabbit's eyes, liver, spleen, lungs, brain, kidneys, heart, and adrenal glands were taken out for sectioning and microscopic study. Four weeks after animal inoculation of toxoplasma gondii, the same procedure was carried out. In the experimental approach to the problem of animal inoculation of toxoplasma gondii, the results will be described as follows: 1. In intraocular animal inoculation, the choroid and uvea showed numerous typical small round basophilic bodies supposed to be toxoplasma gondii and phagocytosed giant epithelial cells. 2. Four weeks after animal intraocular inoculation, the liver and spleen especially R.E.S. system showed a significant toxoplamic findings. 3. In comparison with one week and four weeks after animal inoculation, showed clinically and histologically more severe inflammatory changes in the former than those in the latter.

MeSH Terms

Adrenal Glands
Animals*
Animals, Wild
Anterior Chamber
Basophils
Brain
Chorioretinitis
Choroid
Diagnosis
Epithelial Cells
Gyeonggi-do
Heart
Humans
Inflammation
Kidney
Korea
Liver
Lung
Ophthalmoscopes
Parasites
Spleen
Toxoplasma*
Uvea
Uveitis