Korean J Dermatol.  1968 Jul;6(1):1-17.

Experimental Studies on Tissue Mast Cell Changes in Relation to Effects of Various Neurotic Agents

• Lee YJ

Abstract

With the artificially applied local inflammatory stimulus by ultraviolet irradiation to the inner aspect of the left albino rat thigh skin, the tissue mast cell changes were observed for the nerve effectiveness on the irradiated thigh (Lt.) and non-irradiated opposite thigh (Rt.) and tongue. Using the distilled water (for control group), Bistrium Bromide blocking agent), Yhenobarbital Sodium (sedative and hypnotic) and (autonomic ganglion Reserpine (tranquillizer) were regularly subcutaneously injected every 8 hours, and the following results of most cell changes were observed. 1) Without ultraviolet ray irradiation, the rats of first group were received distilled water and average number of mast cells at the Rt. and Lt. inner aspects of thigh and tongue were gradually decreased and 24 hours after injection, the number of each part showed lowed curve, that was 85. 1%, 84. 8% and 91. 8% of normal average number. On the second and 4th day of the injection, increasing number of each part reached to the normal average number. The 7th day of injection maximum number of above average was l02.1%, 110.8% and 110.7% 2) With the ultraviolet ray irradiation the rats of second group received distilled water. At the Rt, and Lt. inner aspects of the thigh and tongue, the average number of mast cells gradually decreased and on the 4th day of the injection, the lowest number was 59.3,"o, 78.7% and 86. 2% of each average number. However on the 7th day the increasing number showed 75.0%, 84.6% and 89.6% of average number. 3) Group 3; Received Bistrium Bromide injection respectively, 24 hours after injection at the Rt. and Lt. inner aspects of the thigh the gradually decreasing number of mast cells showed the lowest curve, it accounted 70.6% and 74.0% of normal value. However at the tongue it was 77.2% on the second day of injection. Their highest number was seen on the 7th day, it was 95.7%, 100.5% and 107. 8% respectively 4) Group 4; Received Bistrium Bromide and ultraviolet ray irradiation, the average number of mast cells at the Rt. and Lt. inner aspects of the thigh and tongue showed decreasing curve. On the second day of injection the lowest number was 43.3%, 65.4% and 74.9/o of normal value respectively. On the 4th day of injection and irradiation the average number was increased, then after 7th day of injection maximum value was 93. 7%, 92.1% and 115.1% of normal number. 5) Group 5; Received Phenobarbital Sodium, the average number of mast cells at the Rt. and Lt. inner aspects of the thigh and tongue was gradually decreased, the lowest number was seen on the 24 hours after injection, it was 76.7%, 8.7% and 90. 9% of normal value. On the second and 4th day of injection, the curve was increased and on the 7tIi day, the highest value was 106.5%, 104.8% and 104. 6% of normal average value. 6) Group 7; Received Phenobarbital Sodium with ultraviolet rayirradiation at the Rt. and Lt. inner aspects of the thigh and tongue, the average number of must cells were gradually decreased. On the 4th day of injection and irradiation, they reached to the lowest number, and it was 43.3%, 80.6% and 74.0% of normal number. On the 7th day it showed the highest value of 74.9%, 97.2% and 95.8% respectively. 7) Group 7; Received Reserpine at the Rt. and Lt. inner aspect; of the thigh and tongue, the number of mast cells gradually decreased. On the 4 hours after injection 85.4%, 84.0% and 87.9% of average normal number was seen. On the second day, respectively number was increased. On the 7th day maximum increasing number was 103.1%, 115.2% and 95.0% of normal average number 8) Group 8; Received Reserpine and ultraviolet ray irradiation, the decreasing number of mast cells reached to the lowest value on the 4th day after injection, it was 53.5%, 80.3% and 84. 4% of normal value. On the 7th day of procedure, increased number showed 94.8%, 110.3% and 92.9 % of normal value. From the above experimental study, Ristrium Bromide, Phenobarhital Scdium and Reserpibne gave some effect to the change of mast cell at the irracliaticn area by ultraviolet ray and non-irradiated distant part.

MeSH Terms

Animals
Ganglion Cysts
Mast Cells*
Phenobarbital
Rats
Reference Values
Reserpine
Skin
Sodium
Thigh
Tongue
Ultraviolet Rays
Water
Phenobarbital
Reserpine
Sodium
Water