Korean J Leg Med.  1997 Oct;21(2):21-37.

Isolation of polymorphic loci from the human genome using multi-locus probe pV47-2

• Nam YS
• Lee HR
• Han GR
• Hwang JJ

Abstract

Two polymorphic loci, so- called FS106 and FS185, have been isolated from the human genome, using a multilocus probe pV47-2, which is extensively used in Korea for forensic investigation such as resolving paternity disputes. Among the several plaques selected from lambda Fix-II genomic libraries, fourteen clones have been characterized. Restriction maps of 14 clones were constructed to define the flanking as well as repeat parts. The repeat-free flanking DNA fragments were tested for single locus specific polymorphism, and repeat containing DNA fragments were sequenced for the design of PCR primers. None of the repeat-free flanking DNA fragments was not shown any polymorphisms by RFLP analysis. The (GGT)(n)-rich sequences in most of repeat containing DNA fragments were identified by sequencing analysis. Most of repetitive sequences consists of major units of (GGT)(n), but a regular repetition pattern can not be found in all clones. Two sets of primers designed from flanking sequences of repeat containing DNA fragments were shown length polymorphisms by PCR analysis, when tested in 50 unrelated individuals. Three and four alleles were detected at FS106 and 0.58 for FS185. In addition, two loci, FS106 and FS185, have been mapped on chromosome 5 and 3, respectively, by somatic cell hybrid analysis.

MeSH Terms

Alleles
Chromosomes, Human, Pair 5
Clone Cells
Dissent and Disputes
DNA
Genome, Human*
Genomic Library
Humans
Humans*
Hybrid Cells
Korea
Paternity
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Repetitive Sequences, Nucleic Acid
DNA