J Korean Soc Plast Reconstr Surg.  2011 Nov;38(6):725-732.

Animal Skin Pigmentation Model Using Full Thickness Skin Graft in C57BL/6 Mouse

• Lee HK
• Park JL
• Heo EJ
• Kim S

Abstract

PURPOSE
Skin grafting is one of the most commonly used methods in reconstructive plastic surgery field, but complications such as color change, contracture or hypertrophy are common problems. However, pathophysiology of the color change after skin graft is not yet determined and no animal model is established.
METHODS
Full thickness skin grafts were performed on the dorsum of C57BL/6 mice. Serial chronological gross inspection for color change and pigmentation were examined. Melanin pigments were traced by Fontana-Masson staining and semi-quantitative analysis was performed. In addition, immunohistochemical staining of S-100, Micropthalmia related Transcription Factor(MITF) and Melan-A antibodies were also performed to observe melanocytes and their changes.
RESULTS
After skin graft, color change and pigment spots were observed in the graft. Fontana-Masson staining showed melanin pigments in the epidermal and dermal layers in all mice. Immunohistochemistry staining to S-100, MITF, Melan-A antibodies showed melanocytes at the basal layer of epidermis and dermis.
CONCLUSION
In conclusion, we have established an animal model for skin pigmentation after skin graft. We believe this study may be useful in understanding of the behavior of melanocytes after skin graft.

Keyword

C57BL/6 mouse; Skin graft; Pigmentation; Melanocyte; Premature melanocyte

MeSH Terms

Animals
Antibodies
Contracture
Epidermis
Hypertrophy
Immunohistochemistry
MART-1 Antigen
Melanins
Melanocytes
Mice
Models, Animal
Pigmentation
Skin
Skin Pigmentation
Skin Transplantation
Surgery, Plastic
Transplants
Antibodies
MART-1 Antigen
Melanins