J Clin Pathol Qual Control.
1997 Dec;19(2):335-343.
Evaluation of the A TB 32C System for Identification of Candida species
- Affiliations
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- 1Department of Clinical Pathology, Chonnam University Medical School and Chonnam University Hospital, Kwang Ju, Korea.
Abstract
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BACKGROUND: Candida spp. cause a rising incidence of severe infections and the correct identification of Candida spp. is essential for the decision of the clinical significance of a particular isolate as well as the targeted antifungal therapy. We evaluated the ATB 32 system (bioMerieux, France), in comparison with the API 20C system, for the identification of Candida spp, recently isolated from blood cultures.
METHODS
A total of 78 Candida spp. (24 C. tropicalis, 20 C. parapsilosis, 15 C. albicans, 7 C. pelliculosa, 4 C. krusei, 3 C. lipolytica and 5 others) isolated from the blood specimes were identified using ATB 32C system. The results were compared with those of API 20C (bioMerieux, France). Isolates that could not be identified with both system or which showed discrepant results in two systems were identified by germ tubetest, cornmeal agar morphology, CHROMagar Candia or/and DNA probe assay.
RESULTS
Both the ATB 32C and the API 20C correctly identified 67 (86%) of 78 Candida spp.. Eighteen (75%) C. tropicalis and 18 (90%) C. parapsilosis were correctly identified with ATB 32C while 23 (96%) C. tropicalis and 20 (100%) C. parapsilosis were correctly identified with the API 20C. The ATB 32C correctly identified 7 (100%) of C. pelliculosa, 3 (100%) C. lipolytica and 3 (75%) of C. krusei. The API 20C, however, correctly identified 6 (67%), 0 (0%), and 0 (0%), respectively. When the additional idetification with API 20C or CHROMagar was performed for 11 isolates that could not be identified with ATB 32C, total identification rate was increased to 97% (76/78).
CONCLUSIONS
Although it has a limitation to identify C. tropicalis, the ATB 32C system is a useful method for identification of Candida spp., especially by the aid of API 20C or CHROMagar Candida.
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