Korean J Dermatol.  2005 Oct;43(10):1343-1348.

Identification of Candida Species Using CHROMagar Candida in Superficial Cutaneous Candidiasis

Affiliations
  • 1Department of Dermatology, College of Medicine, Dongguk University, Gyeongju, Korea.
  • 2Department of Laboratory Medicine, College of Medicine, Dongguk University, Gyeongju, Korea. mksuhmd@hanmail.net

Abstract

BACKGOUND: CHROMagar Candida is a new differential culture medium that allows selective isolation and identification of clinically important Candida species. However, no study of CHROMagar Candida in superficial cutaneous candidiasis has been reported in Korea. OBJECTIVE: The purpose of this study was to evaluate the use of CHROMagar Candida to identify Candida species isolated from patients with cutaneous candidiasis. METHOD: A total of 95 strains isolated from 92 patients with candidiasis (70 Candida albicans, 9 Candida parapsilosis, 7 Candida guilliermondii, 1 Candida krusei, 1 Candida glabrata, 1 Candida tropicalis, 2 C. albicans plus C. parapsilosis, 1 C. albicans plus C. krusei) were subcultured to CHROMagar Candida (KOMED, Korea) and incubated for 48 hours. Colony appearance on CHROMagar Candida was assessed by two observers. RESULTS: Expected colony appearance on CHROMagar Candida was 100% for C. albicans, C. krusei, C. glabrata and C. tropicalis, respectively but 85.7% for C. guilliermondii and 77.8% for C. parapsilosis. Three mixed cultures of Candida species, not detected by conventional methods, were detected by CHROMagar Candida. CONCLUSION: CHROMagar Candida is a useful isolation medium capable of a rapid presumptive identification of Candida species and more reliable detection of mixed cultures in clinical specimens.

Keyword

CHROMagar Candida; Superficial cutaneous candidiasis

MeSH Terms

Candida albicans
Candida glabrata
Candida tropicalis
Candida*
Candidiasis
Candidiasis, Cutaneous*
Humans
Korea
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