J Korean Neurosurg Soc.  2005 Oct;38(4):293-298.

p53 Protein Expression Area as a Molecular Penumbra of Focal Cerebral Infarction in Rats

Affiliations
  • 1Department of Neurosurgery, College of Medicine, Chung-Ang University, Seoul, Korea. nshhj@hanmail.net

Abstract


OBJECTIVE
The authors investigate the spatial characteristics of apoptotic genes expressed around the focal cerebral infarction, and attempted to explain the penumbra with them. METHODS: A delayed focal cerebral infarction was created in twelve adult Sprague-Dawley rats. We performed the immunohistochemical staining for the apoptosis, bcl-2 and p53 proteins and measured the local cerebral blood flow (CBF) at the infarction core area and peri-infarct area pre- and intra-operatively. The peri-infarct area was divided into six sectors by distance from the infarction border. RESULTS: The size (mm2) of apoptosis, bcl-2, and p53 areas were 3.1+/-1.2, 4.7+/-2.1, and 6.8+/-2.4, respectively. Apoptosis, bcl-2 or p53 positive cells were concentrated at the peri-infarct area adjacent to the infarction core. Their numbers reduced peripherally, which was inversely proportional to the local CBF. The p53 area seems to overlap with and larger than the ischemic penumbra. CONCLUSION: The p53 positive area provides a substitutive method defining the penumbra under the molecular base of knowledge.

Keyword

Infarction; Penumbra; Cerebral blood flow; Apoptosis p53

MeSH Terms

Adult
Animals
Apoptosis
Cerebral Infarction*
Humans
Infarction
Rats*
Rats, Sprague-Dawley
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