J Korean Orthop Res Soc.  2002 Apr;5(1):61-68.

The Effect of High Dose Methotrexate loaded Acrylic Cement in Type II Collagen Induced Arthritis in Rats

• Son JH
• Jung GH
• Kim JD
• Chung SH
• Cho MR

Abstract

PURPOSE: We had examined the effect of high dose MTX loaded acrylic cement in the treatment of type II collagen induced arthritis.
MATERIALS AND METHODS
we used inbreed rat(16weeks - 20weeks) and divided into control group and experimental groups. inbreed rat was induced arthritis by prepared collagen through injection at the tail base. The control group was injected with collagen only. but the experimental groups were inserted intraperitoneally MTX-loaded acryl cement: 25mg, 20mg, 15mg, and 10mg MTX in acrylic cement (0.5gm) respectively. The histopathologic results were investigated to determine the effect of MTX on the 3rd, 7th, 14th, 28th, 42th, and 56th day after inserting high dose MTX loaded acrylic cement which was inserted intraperitoneally 2weeks later of collagenase injection
RESULTS
The arthritis index of the MTX-loaded groups had reduced than arthritis index of the control group according to the dosage and In histopathological test, the experimental group injected with 15mg, 20mg, and 25mg of MTX had distinctive effects, compared to the control group, as dosage chronologically.
CONCLUSION
The treatment of high-dose MTX loaded acrylic cement might be effective on rheumatoid arthritis managment.

Keyword

Type II collagen induced arthritis; High-dose methotrexate; Acrylic cement

MeSH Terms

Animals
Arthritis*
Arthritis, Rheumatoid
Collagen
Collagen Type II*
Collagenases
Methotrexate*
Rats*
Tail
Collagen
Collagen Type II
Collagenases
Methotrexate