J Korean Soc Spine Surg.  2002 Mar;9(1):1-7. 10.4184/jkss.2002.9.1.1.

Macrophage Infiltrations and Expressions of Matrix Metallproteinases in Intervertebral Disc

• Jeon CH
• Ha SY
• Kim HK

• KMID: 1897083
• DOI: http://doi.org/10.4184/jkss.2002.9.1.1

Abstract

STUDY DESIGN: Immunohistologic staining of Intervertebral discs at surgery was performed using CD68, matrix metallopro-teinase-1(MMP-1) and matrix metalloproteinase-3(MMP-3).
OBJECTIVES
1. To investigate the histologic characteristics of the macrophage infiltrations in herniated discs and degenerative discs. 2. To investigate the difference of immumoexpression of MMP-1 and MMP-3 activity to herniated discs and degenerative discs. 3. To investigate the possible correlation between the macrophage infiltrations, MMP-1 and MMP-3 in herniated discs and degenerative discs. SUMMARY OF LITERATURE REVIEW: Spontaneous absorption of herniated disc is associated with infiltrating macrophages and matrix metalloproteinases. Matrix metalloproteinases(MMP) are main matrix degradative enzymes in intervertebral disc.
MATERIALS AND METHODS
Sixty-seven specimens of degenerative disc specimens and one hundred and two specimens of lumbar disc herniation were immunostained with monoclonal antibodies to CD68, anti-MMP-1, and anti-MMP-3.
RESULTS
The granulation tissue containing many CD68-positive macrophages was commonly observed in herniated disc and not in degenerative disc. The immunoexpression of MMP-1 and MMP-3 were observed in herniated and degenerative disc and there were the differences of intensity and localization of MMP-1 and MMP-3 expression in herniated disc and not in degenerative disc. There was no correlation between the macrophage infiltrations, MMP-1 and MMP-3 expression.
CONCLUSIONS
The macrophage infiltrations were specific in the granulation tissue of herniated discs. The differents patterns of immunoexpressions of MMP-1 and MMP-3 were observed in herniated and degenerative discs.

Keyword

Intervertebral disc; Macrophage; Matrix metalloproteinase

MeSH Terms

Absorption
Antibodies, Monoclonal
Granulation Tissue
Intervertebral Disc Displacement
Intervertebral Disc*
Macrophages*
Matrix Metalloproteinases
Antibodies, Monoclonal
Matrix Metalloproteinases

Figure

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  Fig. 1. Prominent infiltration in macrophage in herniated disc tissue.(x200)

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  Fig. 2. Immunostain for MMP-1 in herniated disc tissue.(x200)

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  Fig. 3. Immunostain for MMP-3 in herniated disc

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  Fig. 4. MMP-1 positive cell is observed in degenerated disc tissue.(x400)

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  Fig. 5. MMP-3 positive cell is observed in degenerated disc tissue.(x200)

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