J Lab Med Qual Assur.  2004 Jun;26(1):47-69.

Annual Report on External Quality Assessment in Immunoserology in Korea (2003)

• Cha YJ
• Kwon SY
• Kum DG
• Kim SW
• Kim TY
• Kim JR
• Kim HS
• Park MH
• Park SH
• Park AJ
• Son HC
• Yang JS
• Lee KS
• Chae SL

Abstract

The followings are the results for external quality assessment (EQA) in immunoserology for 2003: 1.Evaluation of EQA was done in 2 trials in May and November, about 99% of laboratories participating average 8.2 items. 2.In C-reactive protein (CRP), rheumatoid factor (RF) and anti-streptolysin O (ASO) tests, about 63%, 49% and 44% of the participating laboratories respectively have used quantitative assays. Because the laboratories using quanitiative assays were on the increase annually, commercial control, Liquicheck(TM) Immunology Contol from Bio-Rad Laboratories (Irvine, CA, USA) was used to assure the quality of quantitiavie results in 2003. A few laboratories reproted the outlier results, comparing with the reference ranges presented by the company. 3.Over 92% of participating laboratoreis have used imunoassays including enzyme immunoassay (EIA), microparticle EIA (MEIA), chemiluminescence immunoassay (CIA), immunochromatography assay (ICA) or radioimmunoassay (RIA) for detedting viral antigens or antibodies. Especially for anti-HCV, over 98% of participating laboratoreis have used various kind of imunoassays. Laboratories using ICA increased and about 24% of participating laboratoreis have used ICA for anti-HCV and anti-HIV. However, many laboratories using ICA for detecting anti-HCV reported false negative results, suggesting lower sensitivity of ICA than those of other immunoassays. 4.The criteria of interpretation were considered to be evaluated in Widal test and laboratories using ICA increased in serological tests for syphilis.

Keyword

Immunoserology; External quality assessment; HBsAg; Anti-HBs; Anti-HCV; Anti- HIV; CRP; RF; ASO; Widal; STS; ICA; CIA; EIA; MEIA; Nephelometry; TIA

MeSH Terms

Allergy and Immunology
Antibodies
Antigens, Viral
C-Reactive Protein
Hepatitis B Surface Antigens
Immunoassay
Immunochromatography
Immunoenzyme Techniques
Korea*
Luminescence
Nephelometry and Turbidimetry
Radioimmunoassay
Reference Values
Rheumatoid Factor
Serologic Tests
Syphilis
Antibodies
Antigens, Viral
C-Reactive Protein
Hepatitis B Surface Antigens
Rheumatoid Factor