Korean J Lab Med.  2006 Dec;26(6):431-435. 10.3343/kjlm.2006.26.6.431.

Comparison of Three Assay Systems for Qualitative and Quantitative Results of Hepatitis B Surface Antibody

Affiliations
  • 1Department of Laboratory Medicine, Sanggye Paik Hospital, Inje University College of Medicine, Seoul, Korea. bmshin@unitel.co.kr

Abstract

BACKGROUND: With a technical improvement of the assay system, automated immunoassay analyzers for hepatitis B surface antibody (anti-HBs) are widely used. However, some discrepancies between assays are still being reported. We compared the qualitative and quantitative results of three kinds of anti-HBs assays.
METHODS
Serum samples were collected from 517 patients and anti-HBs were determined using AxSYM AUSAB, Bayer ADVIA Centaur, and Roche Elecsys assay systems.
RESULTS
The concordance rates between the three assays were 95.1% (543/571). The concordance rates were 97.7% between Centaur and Elecsys, 96.3% between AxSYM and Centaur, and 95.6% between AxSYM and Elecsys. Their correlation coefficients for quantitative results were 0.97, 0.94, and 0.93 in the same order. Twenty-eight specimens showed discrepant results, and all of them had antibody values below 31.5 mIU/mL.
CONCLUSIONS
Three immunoassays for anti-HBs presented a high concordance and correlation; however, the results should be interpreted with caution, because there were still significant differences between assay methods, especially for a low-level of anti-HBs.

Keyword

Hepatitis B virus; Anti-HBs; Automated immunoassay

MeSH Terms

Hepatitis B virus
Hepatitis B*
Hepatitis*
Humans
Immunoassay

Figure

  • Fig. 1. Regression graphs for anti-HBs (mIU/mL) excluding the results above 1,000 mIU/mL. (A) Elecsys vs Centaur, (B) AxSYM vs Elecsys, (C) AxSYM vs Centaur.


Reference

References

1. Cha YJ, Kwon SY, Kum DG, Kim SW, Kim TY, Kim JR, et al. Annual report on external quality assessment in immunoserology in Korea (2003). J Lab Med Qual Assur. 2004; 26:47–69.
2. Davidson M, Krugman S. Recombinant yeast hepatitis B vaccine compared with plasma-derived vaccine: immunogenicity and effect of a booster dose. J Infect. 1986; 13:S31–8.
Article
3. Player VA, White D. Comparison of an ELISA system for the quantification of hepatitis B antibody with an automated and a semiautomated system. J Virol Methods. 1993; 45:67–72.
Article
4. Whang DH, Shin BM. Comparison of four-assay system for the quantification of hepatitis B surface antibody. Korean J Lab Med. 2002; 22:424–30.
5. Heijtink RA, Schneeberger PM, Postma B, Crombach W. Anti-HBs levels after hepatitis B immunisation depend on test reagents: routinely determined 10 and 100 IU/l seroprotection levels unreliable. Vaccine. 2002; 20:2899–905.
Article
6. McCartney RA, Harbour J, Roome AP, Caul EO. Comparison of enhanced chemiluminescence and microparticle enzyme immunoassay for the measurement of hepatitis B surface antibody. Vaccine. 1993; 11:941–5.
Article
7. Whang DH, Um TH. Comparison of immunochromatography assays and quantitative immunoassays for detecting HBsAg and anti-HBs. Korean J Lab Med. 2005; 25:186–91.
8. Oh JH, Kim TY, Yoon HJ, Min HS, Lee HR, Choi TY. Evaluation of Genedia HBsAg Rapid Genedia Anti-HBs Rapid for the screening of HBsAg and Anti-HBs. Korean J Clin Pathol. 1999; 19:114–7.
9. Taylor P, Pickard G, Gammie A, Atkins M. Comparison of the AD-VIA Centaur and Abbott AxSYM immunoassay systems for a routine diagnostic virology laboratory. J Clin Virol. 2004; 30:S11–5.
Article
10. Ostrow DH, Edwards B, Kimes D, Macioszek J, Irace H, Nelson L, et al. Quantitation of hepatitis B surface antibody by an automated microparticle enzyme immunoassay. J Virol Methods. 1991; 32:265–76.
Article
11. Doche C, Thome M, Dimet I, Bienvenu J. Evaluation of the fully automated Cobas Core enzyme immunoassay for the quantitation of antibodies against hepatitis B virus surface antigen. Eur J Clin Chem Clin Biochem. 1996; 34:365–8.
12. Chen Y, Wu W, Li LJ, Lou B, Zhang J, Fan J. Comparison of the results for three automated immunoassay systems in determining serum HBV markers. Clin Chim Acta. 2006; 372:129–33.
Article
Full Text Links
  • KJLM
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr