Korean J Lab Med.  2006 Dec;26(6):431-435. 10.3343/kjlm.2006.26.6.431.

Comparison of Three Assay Systems for Qualitative and Quantitative Results of Hepatitis B Surface Antibody

• Yoo SJ
• Oh HJ
• Shin BM

• KMID: 1889822
• DOI: http://doi.org/10.3343/kjlm.2006.26.6.431

Abstract

BACKGROUND: With a technical improvement of the assay system, automated immunoassay analyzers for hepatitis B surface antibody (anti-HBs) are widely used. However, some discrepancies between assays are still being reported. We compared the qualitative and quantitative results of three kinds of anti-HBs assays.
METHODS
Serum samples were collected from 517 patients and anti-HBs were determined using AxSYM AUSAB, Bayer ADVIA Centaur, and Roche Elecsys assay systems.
RESULTS
The concordance rates between the three assays were 95.1% (543/571). The concordance rates were 97.7% between Centaur and Elecsys, 96.3% between AxSYM and Centaur, and 95.6% between AxSYM and Elecsys. Their correlation coefficients for quantitative results were 0.97, 0.94, and 0.93 in the same order. Twenty-eight specimens showed discrepant results, and all of them had antibody values below 31.5 mIU/mL.
CONCLUSIONS
Three immunoassays for anti-HBs presented a high concordance and correlation; however, the results should be interpreted with caution, because there were still significant differences between assay methods, especially for a low-level of anti-HBs.

Keyword

Hepatitis B virus; Anti-HBs; Automated immunoassay

MeSH Terms

Hepatitis B virus
Hepatitis B*
Hepatitis*
Humans
Immunoassay

Figure

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  Fig. 1. Regression graphs for anti-HBs (mIU/mL) excluding the results above 1,000 mIU/mL. (A) Elecsys vs Centaur, (B) AxSYM vs Elecsys, (C) AxSYM vs Centaur.

Reference

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