Korean J Obstet Gynecol.  2001 Jul;44(7):1225-1231.

Effect of human hydrosalpinx fluid on mouse in in vitro fertilization and embryo development

• Yang HS

Abstract

OBJECTIVE
To ascertain if hydrosalpinges are associated with reduced pregnancy rates and increased pregnancy loss after IVF-ET, a study was conducted. Increased volume and leakage of hydrosalpinx fluid may exert negative effects on follicular development and embryo quality and/or render the uterine environment hostile to embryogenesis. This study was undertaken to examine the effect of hydrosalpinx fluid on mouse embryogenesis in vitro.
METHODS
The study was designed by comparison of mouse embryo blastulation rate in media containing increasing concentrations of hydrosalpinx fluid. In vitro fertilization rate and the development of one-, two-, four-cell mouse embryos in medium containing 0% (Control), 0.3%, 0.6%, 0.9%, 5%, 10% and 20% of human hydrosalpinx fluid-containing media was observed.
RESULTS
In vitro fertilization and culture of mouse embryo at 0% (control), 0.3%, 0.6%, 0.9%, 5%, 10%, 20% hydrosalpinx fluid concentrations demonstrated significantly lower blastulation rate at each level compared with the controls (p<0.05). In vivo fertilization and culture of embryo at the same increasing concentration of hydrosalinx fluid showed the same results (p<0.05). And the difference of each embryo development (zygote, 2cell, 4cell) was not significant.
CONCLUSION
Hydrosalpinx fluid is highly embryotoxic. Procedures such as salpingectomy or proximal tubal occlusion to circumvent the passage of hydrosalpinx fluid into the uterine cavity may have beneficial effects on the developmental environment for embryos in vivo.

Keyword

Hydrosalpinx fluid; In vitro fertilization; Embryotoxicity

MeSH Terms

Animals
Embryonic Development*
Embryonic Structures*
Female
Fertilization
Fertilization in Vitro*
Humans*
Mice*
Pregnancy
Pregnancy Rate
Salpingectomy
Sterilization, Tubal