Bacteriological and Immunological Studies of Urinary Tract Infections

lKim, Hui Kyo; Suh, Sung Taik

Abstract

In order to study the change in bacterial population, the effect of immunity on bacteriuria, and the antibody response in urinary tract infections. Escherichia coli 06(06) was inoculated in bladder and kidney of rabbits non-immunized and immunized with 0 antigen of 06 and enterobacterial common. antigen(CA) of Kunin derived from culture supernatant of Salmonella typhimurium. The results obtained are as follow: 1. Rabbits immunized with O6 antigen and CA showed high antibody titers in sera, and tires were usually higher in rabbits immunized with 06 antigen than those with CA. 2. Bacterial counts reached highest number at the first day of observation in urine of rabbits inoculated in bladder, and after 3 days in rabbits inoculated in kidney. Numbers of bacteria decreased gradually after the highest: peak of bacterial counts, but still remained at considerable numbers after 13~l6 days of inoculation. There were no significant differences in bacterial counts between immunized and non-immunized rabbits. 3. Among non-immunized rabbits, antibody titer began to rise from 4th to 7th days of inoculation in bladder, and from 4th day of inoculation :in kidney. Titers were very low in groups inoculated in bladder, and significantly high in rabbits inoculated in kidney. Antibody titers were usually high when large size of inoculum was used 4. Antibody titers decreased ;gradually and slightly when 06- or CA-immunized rabbits were inoculated with 06. 5. Rabbits inoculated 06 into kidney showed considerable increase in antibody titers from 4 to l0days after inoculation in CA. immunized rabbits, while gradual decrease from the beginning of inoculation in those inoculated in bladder. 6. There were no significant changes other than chronic inflammation in kidneys of rabbits inoculated with 06 in kidney of immunized and non-immunized rabbits when observed after about two weeks of inoculation, No significant difference by the size of inoculum was also observed.