Korean J Thorac Cardiovasc Surg.  2004 Mar;37(3):220-227.

In Vivo Experiment of Tissue-Engineered Artificial Vessel

• Lim SH
• Cho SW
• Hong YS
• Kim BS
• Yoo KJ
• Chang BC
• Choi CY

Abstract

BACKGROUND: The number of patients with coronary artery disease and peripheral vascular disease are increasing, and the need of small diameter vessel is also increasing. We developed small diameter artificial vessel and experimented in vivo. MATERIAL AND METHOD: We got allogenic valve from mongrel dogs, and removed all cells from the allogenic valve. Then, we seeded autologous bone marrow cells onto the decellularized scaffold. After implantation of artificial vessel into the canine carotid artery, we performed angiography regularly. In case of vessel occlusion or at 8 weeks after operation, we euthanized dogs, and retrieved the implanted artificial vessels. RESULT: Control vessels were all occluded except one (which developed aneurysmal dilatation). But autologous cell seeded vascular graft were patent by 4 weeks in one, by 6 in one and by 8 weeks in two. Histologic examination of patent vessel revealed similar structure to native artery.
CONCLUSION
Tissue-engineered vascular graft manufactured with decellularized allogenic matrix and autologous bone marrow cells showed that tissue engineered graft had similar structure to native artery.

Keyword

Blood vessel prosthesis; Tissue engineering; Graft

MeSH Terms

Aneurysm
Angiography
Animals
Arteries
Blood Vessel Prosthesis
Bone Marrow Cells
Carotid Arteries
Coronary Artery Disease
Dogs
Humans
Peripheral Vascular Diseases
Tissue Engineering
Transplants