Korean J Thorac Cardiovasc Surg.  2000 Apr;33(4):277-284.

The Effect of Platelet Derived Growth Factor on the Myocardial Revascularization in Implantation of Rabbit Internal Mammary Artery

• Kim YH
• Rha SJ
• Jung SH
• Moon SW
• Jo KH
• Wang YP
• Kim SW
• Kwak MS

Abstract

BACKGROUND: Coronary artery bypases grafting in the old aged is associated with high mortality and morbidity, and it is difficult to perform if the coronary artery is diffusely disease. Recently it has been known that platelet derived growth factor(PDGF), especially PDGF-BB, stimulates angiogenesis. MATERIAL AND METHOD: New Zealand white rabbit were used. In an attempt to achieve effevtive cardiac revasculatrization without vascular anastmosis, we divided into three groups(group I : Left anterior descending artery(LAD) was occluded by ligature, group II : Bilateral internal mammary vascular pedicles were dissected and implanted into myocardium, group III : The vascular pedicles were implanted into myocardium and PDGF-BB was injected into the myocardial tissue). Two weeks after IMA implantation, the proximal region of implanted LAD was ligated. Four days after LAD ligation angiogram, triphenyl tetrazolium chloride(TTD) staining and hematoxylin eosin staining were performed. RESULT: 1. Survival rate in group II was significantly higher than that in group I (P<0.05), and survival rate in group III was signficantly higher than that in group II(53% vs 93%, P<0.01). 2. There were significant differences in the ratio of area of necrosis to area at risk between group I and group II, and between group II and group III (P<0.01). 3. Microangiogram for angiogenic response revealed wide area of extensive revascularization with patent vessels in group III. 4. Histologic findings of three groups showed that polymorphonuclear leukocyte infiltration was minimal in group II and none in group III.
CONCLUSIONS
PDGF-BB can establish functinal cardiac revasculatization through systemic vessels implanted directly into the myocardium.

Keyword

Coronary artery bypass; Myocondial infarction; Internal thoracic artery; Growth factor

MeSH Terms

Blood Platelets*
Coronary Artery Bypass
Coronary Vessels
Eosine Yellowish-(YS)
Hematoxylin
Ligation
Mammary Arteries*
Mortality
Myocardial Revascularization*
Myocardium
Necrosis
Neutrophils
New Zealand
Platelet-Derived Growth Factor*
Survival Rate
Transplants
Eosine Yellowish-(YS)
Hematoxylin
Platelet-Derived Growth Factor