Korean J Anesthesiol.  2003 Jan;44(1):111-115. 10.4097/kjae.2003.44.1.111.

Changes of Hypoxic Pulmonary Vasoconstriction in Isolated Endotoxin-Treated Rat Lungs

• Kim JY
• Kim SD
• Go H

• KMID: 1860698
• DOI: http://doi.org/10.4097/kjae.2003.44.1.111

Abstract

BACKGROUND: Several investigations have studied hypoxic pulmonary vasoconstriction (HPV) during endotoxemia and there is an increase in nitric oxide (NO) in pulmonary vessels. However, these studies yielded conflicting or at times contradictory results, since reference has been made to both enhancement and inhibition of HPV. Our objective was to determine the changes of hypoxic pulmonary vasoconstriction on the isolated blood-perfused lung of endotoxemic rats.
METHODS
Pulmonary arterial pressure (PAP) was measured in a blood-perfused lung preparation from Sprague-Dawley rats in normoxia (21% O2, 5% CO2, balanced N2) and hypoxia (5% O2, 5% CO2, balanced N2). We studied the effect of normoxia and hypoxia in a control group, Escherichia coli lipopolysaccharide group (LPS) and LPS with N omega-nitro-L-arginine methyl ester group (L-NAME).
RESULTS
The Baseline PAP was higher in LPS (15.0+/-4.0 mmHg) compared with control group (10.9+/-2.9 mmHg) and L-NAME (11.1+/-3.6 mmHg). In hypoxia, HPV was higher in L-NAME (109.6+/-100.2%) compared with control group (59.9+/-31.6%) and LPS (58.8+/-33.8%)(P<0.05).
CONCLUSIONS
We conclude that NO is an important factor to impair HPV during endotoxemia.

Keyword

Endotoxemia; hypoxic pulmonary vasoconstriction; L-NAME; lipopolysaccharide; nitric oxide

MeSH Terms

Animals
Anoxia
Arterial Pressure
Endotoxemia
Escherichia coli
Lung*
NG-Nitroarginine Methyl Ester
Nitric Oxide
Rats*
Rats, Sprague-Dawley
Vasoconstriction*
NG-Nitroarginine Methyl Ester
Nitric Oxide