Diabetes Metab J.  2011 Oct;35(5):523-528. 10.4093/dmj.2011.35.5.523.

Serum Visfatin and Fetuin-A Levels and Glycemic Control in Patients with Obese Type 2 Diabetes Mellitus

• Gunduz FO
• Yildirmak
• Temizel M
• Faki Y
• Cakmak M
• Durmuscan
• Sezgin F

• KMID: 1857508
• DOI: http://doi.org/10.4093/dmj.2011.35.5.523

Abstract

BACKGROUND
Visfatin is an adipokine produced by visceral adipose tissue and has insulin-mimicking effects. Fetuin-A is a hepatic secretory protein that binds the insulin receptor and inhibits insulin action both in vivo and in vitro. The authors of the present study aimed to investigate the levels of serum visfatin and fetuin-A and their correlation with hemoglobin A1c (HbA1c) and urine albumin levels in patients with type 2 diabetes mellitus (T2DM).
METHODS
A total of 40 obese patients with T2DM (11 males and 29 females; age, 54.47+/-10.83 years and 23 obese nondiabetic controls (8 males and 15 females; age, 53.04+/-11.33 years) were included in the study. Age, sex, and body mass index were similar in the 2 groups. Serum visfatin and fetuin-A levels were measured by enzyme-linked immunosorbent assay. HbA1c and urine albumin levels were measured by high performance liquid chromatography and nephelometric method, respectively.
RESULTS
Serum levels of visfatin in patients with T2DM (4.03+/-2.44 ng/mL) were similar to the control group (3.65+/-3.02 ng/mL). Serum fetuin-A levels were significantly lower in patients with T2DM than the controls (298.75+/-78.86 and 430.73+/-94.46 microg/mL, respectively). HbA1c levels were significantly higher in the T2DM group compared with controls (7.33+/-1.32 and 5.44+/-0.84%, respectively). Correlations between visfatin, fetuin-A and HbA1c levels were not observed.
CONCLUSION
The present study suggests fetuin-A may play a role in the pathogenesis of T2DM.

Keyword

Albuminuria; Diabetes mellitus, type 2; Fetuin-A; Hemoglobin A1c; Visfatin

MeSH Terms

Adipokines
Albuminuria
alpha-2-HS-Glycoprotein
Body Mass Index
Chromatography, Liquid
Diabetes Mellitus, Type 2
Enzyme-Linked Immunosorbent Assay
Hemoglobins
Humans
Insulin
Intra-Abdominal Fat
Male
Nicotinamide Phosphoribosyltransferase
Receptor, Insulin
Adipokines
Hemoglobins
Insulin
Nicotinamide Phosphoribosyltransferase
Receptor, Insulin
alpha-2-HS-Glycoprotein

Reference

1. Guerre-Millo M. Adipose tissue hormones. J Endocrinol Invest. 2002. 25:855–861.

2. Samal B, Sun Y, Stearns G, Xie C, Suggs S, McNiece I. Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor. Mol Cell Biol. 1994. 14:1431–1437.

3. Ognjanovic S, Bao S, Yamamoto SY, Garibay-Tupas J, Samal B, Bryant-Greenwood GD. Genomic organization of the gene coding for human pre-B-cell colony enhancing factor and expression in human fetal membranes. J Mol Endocrinol. 2001. 26:107–117.

4. Berndt J, Kloting N, Kralisch S, Kovacs P, Fasshauer M, Schon MR, Stumvoll M, Bluher M. Plasma visfatin concentrations and fat depot-specific mRNA expression in humans. Diabetes. 2005. 54:2911–2916.

5. Haider DG, Schaller G, Kapiotis S, Maier C, Luger A, Wolzt M. The release of the adipocytokine visfatin is regulated by glucose and insulin. Diabetologia. 2006. 49:1909–1914.

6. Fukuhara A, Matsuda M, Nishizawa M, Segawa K, Tanaka M, Kishimoto K, Matsuki Y, Murakami M, Ichisaka T, Murakami H, Watanabe E, Takagi T, Akiyoshi M, Ohtsubo T, Kihara S, Yamashita S, Makishima M, Funahashi T, Yamanaka S, Hiramatsu R, Matsuzawa Y, Shimomura I. Visfatin: a protein secreted by visceral fat that mimics the effects of insulin. Science. 2005. 307:426–430.

7. Chen MP, Chung FM, Chang DM, Tsai JC, Huang HF, Shin SJ, Lee YJ. Elevated plasma level of visfatin/pre-B cell colony-enhancing factor in patients with type 2 diabetes mellitus. J Clin Endocrinol Metab. 2006. 91:295–299.

8. Hammarstedt A, Pihlajamaki J, Rotter Sopasakis V, Gogg S, Jansson PA, Laakso M, Smith U. Visfatin is an adipokine, but it is not regulated by thiazolidinediones. J Clin Endocrinol Metab. 2006. 91:1181–1184.

9. Mori K, Emoto M, Araki T, Yokoyama H, Lee E, Teramura M, Koyama H, Shoji T, Inaba M, Nishizawa Y. Effects of pioglitazone on serum fetuin-A levels in patients with type 2 diabetes mellitus. Metabolism. 2008. 57:1248–1252.

10. Ix JH, Wassel CL, Kanaya AM, Vittinghoff E, Johnson KC, Koster A, Cauley JA, Harris TB, Cummings SR, Shlipak MG. Health ABC Study. Fetuin-A and incident diabetes mellitus in older persons. JAMA. 2008. 300:182–188.

11. Yilmaz MI, Saglam M, Qureshi AR, Carrero JJ, Caglar K, Eyileten T, Sonmez A, Cakir E, Oguz Y, Vural A, Yenicesu M, Stenvinkel P, Lindholm B, Axelsson J. Endothelial dysfunction in type-2 diabetics with early diabetic nephropathy is associated with low circulating adiponectin. Nephrol Dial Transplant. 2008. 23:1621–1627.

12. Dogru T, Sonmez A, Tasci I, Bozoglu E, Yilmaz MI, Genc H, Erdem G, Gok M, Bingol N, Kilic S, Ozgurtas T, Bingol S. Plasma visfatin levels in patients with newly diagnosed and untreated type 2 diabetes mellitus and impaired glucose tolerance. Diabetes Res Clin Pract. 2007. 76:24–29.

13. Sandeep S, Velmurugan K, Deepa R, Mohan V. Serum visfatin in relation to visceral fat, obesity, and type 2 diabetes mellitus in Asian Indians. Metabolism. 2007. 56:565–570.

14. Krzyzanowska K, Krugluger W, Mittermayer F, Rahman R, Haider D, Shnawa N, Schernthaner G. Increased visfatin concentrations in women with gestational diabetes mellitus. Clin Sci (Lond). 2006. 110:605–609.

15. Takata H, Ikeda Y, Suehiro T, Ishibashi A, Inoue M, Kumon Y, Terada Y. High glucose induces transactivation of the alpha2-HS glycoprotein gene through the ERK1/2 signaling pathway. J Atheroscler Thromb. 2009. 16:448–456.

16. Mori K, Emoto M, Yokoyama H, Araki T, Teramura M, Koyama H, Shoji T, Inaba M, Nishizawa Y. Association of serum fetuin-A with insulin resistance in type 2 diabetic and nondiabetic subjects. Diabetes Care. 2006. 29:468.