Nucl Med Mol Imaging.  2009 Oct;43(5):451-458.

Development of Quantification Method for Bioluminescence Imaging

Affiliations
  • 1Department of Nuclear Medicine, Chonnam National University Hospital, Hwasun, Korea. dewpapa@hanmail.net
  • 2Department of Physics, Chosun University, Gwangju, Korea.
  • 3Department of Medical Image Science, Inje University, Kimhae, Korea.
  • 4Center for Clinical Vaccine Research, Hwasun, Jeonnam, Korea.

Abstract

PURPOSE
Optical molecular luminescence imaging is widely used for detection and imaging of bio-photons emitted by luminescent luciferase activation. The measured photons in this method provide the degree of molecular alteration or cell numbers with the advantage of high signal-to-noise ratio. To extract useful information from the measured results, the analysis based on a proper quantification method is necessary. In this research, we propose a quantification method presenting linear response of measured light signal to measurement time.
MATERIALS AND METHODS
We detected the luminescence signal by using lab-made optical imaging equipment of animal light imaging system (ALIS) and different two kinds of light sources. One is three bacterial light-emitting sources containing different number of bacteria. The other is three different non-bacterial light sources emitting very weak light. By using the concept of the candela and the flux, we could derive simplified linear quantification formula. After experimentally measuring light intensity, the data was processed with the proposed quantification function.
RESULTS
We could obtain linear response of photon counts to measurement time by applying the pre-determined quantification function. The ratio of the re-calculated photon counts and measurement time present a constant value although different light source was applied.
CONCLUSION
The quantification function for linear response could be applicable to the standard quantification process. The proposed method could be used for the exact quantitative analysis in various light imaging equipments with presenting linear response behavior of constant light emitting sources to measurement time.

Keyword

Photon count; quantification; candela; flux; optical imaging; bioluminescence

MeSH Terms

Animals
Bacteria
Cell Count
Enzyme Multiplied Immunoassay Technique
Imidazoles
Light
Luciferases
Luminescence
Nitro Compounds
Optical Imaging
Photons
Signal-To-Noise Ratio
Imidazoles
Luciferases
Nitro Compounds
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