J Korean Soc Ther Radiol Oncol.
2006 Mar;24(1):67-76.
Effects of Recombinant Human Epidermal Growth Factor (rhEGF) on Experimental Radiation-Induced Oral Mucositis in Rats
- Affiliations
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- 1Department of Toxicology, Daewoong Pharmaceutical Co., LTD., Yongin, Korea.
- 2Department of Pharmacology, Daewoong Pharmaceutical Co., LTD., Yongin, Korea.
- 3Protein Drug Development, Institute of Bioscience and Biotechnology, Daewoong Pharmaceutical Co., LTD., Yongin, Korea.
- 4Department of Plastic Surgery, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
- 5Department of Radiation Oncology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea. lsw@amc.seoul.kr
- 6Department of Radiology, Gyeongsang National University College of Medicine, Jinju, Korea.
Abstract
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PURPOSE: Oral mucositis is a common toxicity of radiation or chemotherapy, which is used a treatment for head and neck cancer. We investigated effects of recombinant human epidermal growth factor (rhEGF) on radiation-induced oral mucositis in rat model.
MATERIALS AND METHODS
Spraque-Dawley rats (7 per group) exposed to a single dose of 25 Gy (day 0) on their head, except for one group, were randomly divided into un-treated, vehicle-treated, and two rhEGF- treated groups. Rats were topically applied with rhEGF (15 or 30 microgram/oral cavity/day) or vehicle to their oral mucosa. Survival rate of rats, weight changes, and food intakes were examined from day 0 to 18 after radiation. Histology study was performed from oral mucosa of rats at day 7 and 18 after radiation.
RESULTS
rhEGF-treated groups (15 or 30 microgram/day) showed all survival rate 33%, whereas un-treated and vehicle-treated groups showed all survival rate 0% at the end of experiment. rhEGF-treated groups statistically had less weight loss compared to vehicle-treated group from day 2 to 7 after radiation. Food intake of rats with rhEGF treatment turned to increase at day 14 after radiation. At 7 day after radiation, un-treated and vehicle-treated groups showed severe pseudomembraneous or ulcerative oral mucositis. On the other hand, rhEGF-treated groups had no more than cellular swelling and degeneration of epidermal cells in oral mucosa of rats.
CONCLUSION
These results suggest that rhEGF has significantly positive effects on radiation-induced oral mucositis in rats. rhEGF display a therapeutic potential on a clinical level.
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