Korean J Urol.
2013 Aug;54(8):547-554. 10.4111/kju.2013.54.8.547.
Possible Role of Sonic Hedgehog and Epithelial-Mesenchymal Transition in Renal Cell Cancer Progression
- Affiliations
-
- 1Department of Urology, Kobe University Graduate School of Medicine, Kobe, Japan. yutoshunta@hotmail.co.jp
- 2Faculty of Medicine, Assiut University, Assiut, Egypt.
- 3Infectious Center, Kobe University Graduate School of Medicine, Kobe, Japan.
- 4Department of Urology, Shinko Hospital, Kobe, Japan.
- 5Department of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
- KMID: 1840469
- DOI: http://doi.org/10.4111/kju.2013.54.8.547
Abstract
- PURPOSE
Sonic hedgehog (Shh) signaling and epithelial-mesenchymal transition (EMT) are both known to relate to cancer progression. The purpose of this study was to investigate the role of Shh signaling and EMT in renal cell carcinoma (RCC).
MATERIALS AND METHODS
Cell proliferation was assayed in RCC cell lines in the presence or absence of a Shh signaling stimulator, recombinant Shh (r-Shh) protein, or a Shh signaling inhibitor, cyclopamine. Real-time reverse transcription-polymerase chain reaction (RT-PCR) was performed to study the expression of EMT markers (E-cadherin, N-cadherin, and vimentin) and osteonectin. The expression of Ki-67, Gli-1, osteonectin, and EMT markers in nephrectomy specimens from RCC patients was also measured by immunohistochemical (IHC) staining.
RESULTS
RCC cells showed enhanced cell proliferation by r-Shh protein, whereas cell proliferation was suppressed by the addition of cyclopamine in RenCa cells. Real-time RT-PCR showed that r-Shh suppressed the expression of E-cadherin and that this suppression was partly blocked by cyclopamine alone in RenCa cells. In the IHC results, osteonectin significantly correlated with vein sinus invasion (p=0.0218), and the expression of vimentin significantly correlated with lymphatic invasion (p=0.0392).
CONCLUSIONS
Shh signaling and EMT play roles in RCC progression, and the Shh signaling inhibitor cyclopamine might be a possible molecular targeted therapeutic strategy for RCC.
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