J Korean Soc Emerg Med.  2007 Oct;18(5):406-413.

Relationships between beta-fibrinogen gene -455 G/A Polymorphism, 448 G/A Polymorphism, and Plasma Fibrinogen Concentration in Korean Ischemic Stroke Patients

• Lee JS
• Cho HJ
• Choi HS
• Hong HP
• Ko YG

Abstract

PURPOSE: The aim of this study was to evaluate the beta-fibrinogen gene -455 G/A and 448 G/A polymorphisms and to determine the relationship of these single nucleotide polymorphisms with plasma fibrinogen concentration and with the development of ischemic stroke in Korea.
METHODS
123 patients who were diagnosed with ischemic stroke at Kyung Hee University Hospital from March 2003 to February 2004 were compared to 230 control subjects. The genotypes of the beta-fibrinogen gene -455 G/A and 448 G/A polymorphisms were confirmed by polymerase chain reaction (PCR), and the allele frequencies of the patient group were compared to those of the control group. The associations of the single nucleotide polymorphisms with development of ischemic stroke and risk factors of ischemic stroke were assessed.
RESULTS
The patient group had more heterozygotes (G/A or A/G) in the beta-fibrinogen gene than did the control group, and statistical analysis of the genotype frequencies indicated a significant difference between the patient group and the control group (p<0.05). The odds ratios of the allele frequencies were 1.53(95% CI; 1.03-2.29) for the -455 G/A polymorphism and 1.85(95% CI; 1.25-2.74) for the 448 G/A polymorphism, indicating a statistically significant difference between the patient and control groups. In ischemic stroke patients, the allele frequencies for smoking history and hyperfibrinogenemia yielded odds ratios of 1.96(95% CI; 1.17-3.27) and 2.40(95% CI; 1.31-4.40), respectively, for the -455 G/A polymorphism and 1.96(95% CI; 1.19-3.22) and 2.42(95% CI; 1.34-4.39) for the 448 G/A polymorphism.
CONCLUSION
The -455 G/A and 448 G/A single nucleotide polymorphisms of the beta-fibrinogen gene appear to be associated with plasma fibrinogen concentration and the development of ischemic stroke in Korea.

Keyword

Stroke; Fibrinogen; Genetics; Single nucleotide polymorphism

MeSH Terms

Fibrinogen*
Gene Frequency
Genetics
Genotype
Heterozygote
Humans
Korea
Odds Ratio
Plasma*
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
Risk Factors
Smoke
Smoking
Stroke*
Fibrinogen
Smoke