Korean J Hematol.  2001 May;36(2):154-161.

Peripheral Blood Stem Cell Collection through Large Volume Leukapheresis after Mobilization with GM-CSF and/or G-CSF in Normal Healthy Donors

• Jung JT
• Sung WJ
• Chae YS
• Seo KW
• Kwon HE
• Park SW
• Lee NY
• Kwak DS
• Kim JG
• Sohn SK
• Suh JS
• Lee KB

Abstract

BACKGROUND
The use of colony stimulating factor (CSF) has increased to mobilize hematopoietic progenitor cells for allo-PBSCT. The most effective mobilization regimen has not yet defined. The authors analyzed the results of the mobilized PBSC collection through large volume leukapheresis from 38 normal healthy donors using three different regimens, namely, a single regimen with GM-CSF (Leucogen ), a concurrent use of GM-CSF and G-CSF (Leucostim ), and a sequential regimen with GM-CSF followed by G-CSF.
METHODS
This study was done on 38 healthy donors from Sep. 1998 to Jan. 2001. One donor was mobilized with G-CSF alone, 9 with GM-CSF alone, 20 with concurrent regimen and 8 with sequential regimen. After 5 days of mobilization treatment, PBSCs were collected by large volume leukapheresis through femoral vein catheter. We compared the results of each collected progenitor cells and observed the side effects.
RESULTS
The average WBC count before apheresis was 22.6+-11.0x103/uL and circulating CD34+cell percent was 1.31+-2.24%. Total 66 times with an average of 1.46+-0.61 of largevolume leukapheresis were performed for the 37 donors. The mean collected MNC count was 4.61+-2.77x108/kg, CD3+cell count was 2.95+-1.82x108/kg and CD34+cell count was 9.76+-12.42x106/kg. A significant side effect observed after large volume leukapheresis was thrombocytopenia showing decrease from 199.1+-52.2 to 80.7+-25.2x103/uL without any bleeding tendency. The mean collected MNC counts provoed to be significantly higher in combination groups with GM-and G-CSF than GM-CSF alone (P<0.05). The CD34+cell counts showed to be statistically higher in a sequential group compared to the concurrent and single regimen groups (P<0.05).
CONCLUSION
A mobilization protocol with combination regimens of GM-CSF and G-CSF seemed to be superior to a single regimen with GM-CSF. Large volume leukapheresis through femoral vein catheter after mobilization with combination regimens of GM-and G-CSF in normal healthy donors was safe and proved to be an excellent. method to harvest stem cells.

Keyword

Allogeneic PBSC; Mobilization

MeSH Terms

Blood Component Removal
Catheters
Colony-Stimulating Factors
Femoral Vein
Granulocyte Colony-Stimulating Factor*
Granulocyte-Macrophage Colony-Stimulating Factor*
Hematopoietic Stem Cells
Hemorrhage
Humans
Leukapheresis*
Stem Cells*
Thrombocytopenia
Tissue Donors*
Colony-Stimulating Factors
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor