J Korean Orthop Res Soc.  2001 Oct;4(2):167-176.

Effects of TGF- beta 3 pretreatment in vitro on the differentiation of rabbit mesenchymal stem cell in vivo

• Park HG
• Park SR
• Lim HJ
• Min BH

Abstract

PURPOSE
Bone and cartilage were manufactured by using tissue engineering of mesenchymal stem cell (MSC) which can differentiate into variety of cell types. MATERIAL AND METHOD: MSC was isolated and cultured from the rabbit weighing 500g, and it was seeded into PGA mesh and pre-cultured for 1 week with different TGF- beta 3 treated conditions. It was implanted into nude mice and tissues generated were recovered from 1, 2, 3, 4, 8 ,and 12 weeks respectively. Degree of bone and cartilage formation was analyzed with histology and immunohistochemistry assay. RESULT: Pre-culture condition with TGF- beta 3 treatment showed early start of chondrogenic differentiation, and degree of bone and cartilage formation was promoted as time passed. But both of the cases differentiated into complete bone after 12 weeks.
CONCLUSION
The results show that pretreatment of TGF- beta 3 promotes the differentiation process in vivo condition under the in vivo system where MSC differentiate into bone via cartilage formation.

Keyword

Chondrogenic differentiation; Rabbit mesenchymal stem cell; Osteogenic differentiation; PGA; Tissue engineering; TGF- beta 3

MeSH Terms

Animals
Cartilage
Immunohistochemistry
Mesenchymal Stromal Cells*
Mice
Mice, Nude
Tissue Engineering