J Korean Ophthalmol Soc.  2004 Feb;45(2):296-302.

Suppression of VEGF by Aminoguanidine in RPE Cells Cultured in the Hyperglycemic Condition

• Kim JS
• Lee IG
• Cho HK

Abstract

PURPOSE
We evaluated whether aminoguanidine could inhibit VEGF mRNA expression in the retinal pigment epithelial cells cultured at various glucose concentrations. METHODS: Human retinal pigment epithelial cells were cultured in the culture media containing 5.5 mM, or 11 mM, or 16 mM glucose for 5 days, or 7 days, or 14 days respectively. To evaluate an inhibitory effect of aminoguanidine on VEGF mRNA expression, 1 micro M, or 3 micro M, or 10 micro M aminoguanidine was added in the culture media. The VEGF mRNA expression was assayed by northern blot analysis. RESULTS: The VEGF mRNA expression of the cultured retinal pigment epithelial cells increased proportionally with media glucose concentration in culture media. At each glucose concentration of the media, VEGF mRNA expression increased with a prolongation of incubation period. An aminoguanidine inhibited the expression of VEGF mRNA by concentration-dependent manner in 5 day and 7 day incubation, but not in 14 day incubation. CONCLUSIONS: The aminoguanidine could inhibit a new vessel formation in the diabetic retina, and be useful for therapeutic or preventive drug in the diabetic retinopathy.

Keyword

Aminoguanidine; Hyperglycemia; RPE; VEGF

MeSH Terms

Blotting, Northern
Culture Media
Diabetic Retinopathy
Epithelial Cells
Glucose
Humans
Hyperglycemia
Retina
Retinaldehyde
RNA, Messenger
Vascular Endothelial Growth Factor A*
Culture Media
Glucose
RNA, Messenger
Retinaldehyde
Vascular Endothelial Growth Factor A