Korean J Parasitol.
2012 Dec;50(4):391-394.
Human Infections with Liver and Minute Intestinal Flukes in Guangxi, China: Analysis by DNA Sequencing, Ultrasonography, and Immunoaffinity Chromatography
- Affiliations
-
- 1Department of Parasitology and Medical Research Institute, Chungbuk National University School of Medicine, Cheongju 360-763, Korea. kseom@chungbuk.ac.kr
- 2Department of Microbiology and Immunology, Eulji University School of Medicine, Daejeon 301-832, Korea.
- 3Department of Parasitology, Korea University College of Medicine, Seoul 136-705, Korea.
- 4Guangxi Center for Disease Prevention and Control, Nanning, Guangxi, China.
Abstract
- The prevalence of liver and intestinal fluke infections was determined by surveying inhabitants of Hengxuan, Fusui, and Shanglin villages which were known to be endemic for liver flukes in Guangxi, China in May 2010. A total of 718 people were examined for helminth eggs by the Kato-Katz thick smear technique, ultrasonography, immunoaffinity chromatography, and DNA sequencing. The overall egg positive rate was found to be 59.6% (28.0-70.6%) that included mixed infections with liver and intestinal flukes. Cases showing higher than 20,000 eggs per gram of feces (EPG) were detected between 1.3% and 16.2%. Ultrasonographic findings exhibited overall 28.2% (72 of 255 cases) dilatation rate of the intrahepatic bile duct. Clonorchis sinensis infection was detected serologically in 88.3% (38 of 43 cases) among C. sinensis egg positive subjects by the immunoaffinity chromatography using a specific antigen for C. sinensis. For differential diagnosis of the liver and intestinal flukes, more precise PCR and nucleotide sequencing for copro-DNA were performed for 46 egg positive cases. Mixed infections with C. sinensis and Metagonimus yokogawai were detected in 8 of 46 egg positive cases, whereas 29 specimens were positive for Haplorchis taichui. Ultrasonographic findings and immunoaffinity chromatography results showed usefulness, even in a limited way, in figuring out of the liver fluke endemicity.
Keyword
MeSH Terms
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Animals
China/epidemiology
Chromatography, Affinity
Clonorchiasis/*epidemiology/parasitology/ultrasonography
Clonorchis sinensis/genetics/immunology/*isolation & purification
Coinfection
DNA, Helminth/chemistry/genetics
Feces/parasitology
Female
Heterophyidae/genetics/*isolation & purification
Humans
Intestines/*parasitology
Liver/*parasitology
Parasite Egg Count
Polymerase Chain Reaction
Prevalence
Sequence Analysis, DNA
Trematode Infections/*epidemiology/parasitology/ultrasonography
DNA, Helminth
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