J Korean Med Sci.  2010 Sep;25(9):1284-1290. 10.3346/jkms.2010.25.9.1284.

Efficacy of Dendritic Cells Matured Early with OK-432 (Picibanil(R)), Prostaglandin E2, and Interferon-alpha as a Vaccine for a Hormone Refractory Prostate Cancer Cell Line

Affiliations
  • 1Department of Urology, College of Medicine, Hallym University, Hallym Sacred Heart Hospital, Anyang, Korea.
  • 2Department of Urology, College of Medicine, University of Ulsan, Asan Medical Center, Seoul, Korea. cskim@amc.seoul.kr
  • 3Department of Urology, Seoul National University, College of Medicine and Seoul National University Hospital Bundang, Seongnam, Korea.

Abstract

Dendritic cells (DCs) are potent antigen-presenting cells. OK432 (Picibanil(R)) was introduced as a potent stimulator of DC maturation in combination with prostaglandin-E2 and interferon-alpha. We compared the efficacy of a DC-prostate cancer vaccine using early-mature DCs stimulated with OK432, PGE2 and INF-alpha (OPA) with that of vaccines using other methods. On days 3 or 7 of DC culture, TNF-alpha (T), TNF-alpha and LPS (TL) or OPA were employed as maturation stimulators. DU145 cells subjected to heat stress were hybridized with mature DCs using polyethyleneglycol. T cells were sensitized by the hybrids, and their proliferative and cytokine secretion activities and cytotoxicity were measured. The yields of early-mature DCs were higher, compared to yields at the conventional maturation time (P<0.05). In the early maturation setting, the mean fusion ratios, calculated from the fraction of dual-positive cells, were 13.3%, 18.6%, and 39.9%, respectively (P=0.051) in the T only, TL, and OPA-treated groups. The function of cytotoxic T cells, which were sensitized with the hybrids containing DCs matured early with OPA, was superior to that using other methods. The antitumor effects of DC-DU145 hybrids generated with DCs subjected to early maturation with the OPA may be superior to that of the hybrids using conventional maturation methods.

Keyword

Dendritic Cells; Prostatic Neoplasms; Cancer Vaccines; Immunotherapy

MeSH Terms

Cancer Vaccines/*immunology
Cell Line, Tumor
Dendritic Cells/cytology/drug effects/*immunology
Dinoprostone/*pharmacology
Humans
Immunologic Factors/*pharmacology
Interferon-alpha/*pharmacology
Lipopolysaccharides/toxicity
Male
Neoplasms, Hormone-Dependent/*immunology
Phenotype
Picibanil/*pharmacology
Prostatic Neoplasms/*immunology
T-Lymphocytes, Cytotoxic/immunology
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