J Korean Med Sci.  2007 Sep;22(Suppl):S47-S51. 10.3346/jkms.2007.22.S.S47.

High Frequency of Genetic Alterations in Non-small Cell Lung Cancer Detected by Multi-target Fluorescence In Situ Hybridization

• Kang JU
• Koo SH
• Kwon KC
• Park JW
• Shin SY
• Kim JM
• Jung SS

• KMID: 1785788
• DOI: http://doi.org/10.3346/jkms.2007.22.S.S47

Abstract

Detection of genetic alterations could provide a tool as an adjuvant for the diagnosis of non-small cell lung cancer (NSCLC) and to define patients at risk for early relapse. In this study, a multi-target fluorescence in situ hybridization (FISH) assay was conducted to investigate the correlation between the alterations of chromosomes, including 5p15.2, 6p11.1-q11, 7p12, and 8q24.12-q24.13 (LaVysion Test), and clinicopathological variables, and to clarify the potential of the multi-target FISH assay in 37 NSCLC. The most notable finding was the higher frequency of a gain in chromosome 5p15.2 in early-stage (I+IIa) lung cancers. The frequency of the gain was 81.3% (16/22) in stage I tumors. The frequencies of gains in 6p11.1-q11 and 8q24.12-q24.13 were 61.5% (8/13) and 84.6% (11/13) in stage IIIa cancers, as compared with lower frequencies in stage I tumors at 25.0% and 31.3%, respectively. There was also a significant difference in the histological type. Our results suggest that a gain in 6p11.1-q11 and 8q24.12-q24.13 plays an important role in tumor progression and is associated with histological differentiation. On the other hand, gene amplification in the 5p region was one of the most consistent alterations in early-stage lung cancer, and thus a series of genes in the critical 5p15.2 region might potentially associated with the development of lung cancer.

Keyword

NSCLC; Genetic Alteration; Multi-target FISH; 5p15.2; 6p11.1-q11; 7p12; 8q24.12-q24.13

MeSH Terms

Aged
Carcinoma, Non-Small-Cell Lung/diagnosis/*genetics/pathology
*Chromosome Aberrations
Chromosomes, Human, Pair 5/genetics
Chromosomes, Human, Pair 6/genetics
Chromosomes, Human, Pair 8/genetics
Female
Gene Amplification
Humans
In Situ Hybridization, Fluorescence
Lung Neoplasms/diagnosis/*genetics/pathology
Male
Middle Aged
Neoplasm Staging
Tumor Markers, Biological/genetics

Figure

• 

  Fig. 1 Representative examples of a multi-color, multi-target FISH assay from patients with lung cancer. (A) Normal control (6p11.1-q11; aqua, 5p15.2; green, 7p12 red, 8q23.12-q24.13; yellow). (B) FISH-positive cells with polysomy of adenocarcinoma (5 red, 4 green, 4 aqua, and 2 yellow signals). (C) FISH-positive cells with polysomy of squamous cell carcinoma (3 red, 1 green, 9 aqua, and 7 yellow signals).

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