J Vet Sci.  2014 Jun;15(2):309-316. 10.4142/jvs.2014.15.2.309.

Accuracy of capillary blood 3-beta-hydroxybutyrate determination for the detection and treatment of canine diabetic ketoacidosis

• Bresciani
• Pietra M
• Corradini
• Giunti M
• Fracassi F

• KMID: 1784653
• DOI: http://doi.org/10.4142/jvs.2014.15.2.309

Abstract

In human medicine, diagnosis of diabetic ketoacidosis (DKA) is usually based on measurement of capillary 3-beta-hydroxybutyrate (3-HB) with a hand held ketone sensor. This study was conducted to determine if measurement of capillary 3-HB could be useful for the diagnosis and monitoring of canine DKA. Fifteen dogs with diabetic ketosis and 10 with DKA were evaluated. Paired measurements of 3-HB of capillary and venous blood samples were analysed by the electrochemical sensor and reference method. Use of capillary 3-HB measurement during DKA management was then evaluated through simultaneous measurements of capillary 3-HB, urinary AcAc and venous blood gas analysis. Good agreement between capillary and venous 3-HB measurement was detected by the electrochemical sensor and reference method. Monitoring treatment of DKA revealed a significant correlation between capillary 3-HB and acidosis markers, while no significant correlation was observed between AcAc and acidosis markers. A cut-off value of capillary blood 3-HB >3.8 mmol/L for diagnosis of DKA resulted in 70% and 92% sensitivity and specificity. The electrochemical sensor accurately measures 3-HB concentration in both capillary and venous blood samples, is accurate in diagnosing canine DKA, and appears to reflect the patient's metabolic status during DKA treatment.

Keyword

acetoacetate; diabetes mellitus; dog; ketone bodies

MeSH Terms

3-Hydroxybutyric Acid/blood/*diagnostic use
Animals
Blood Chemical Analysis/standards/*veterinary
Blood Specimen Collection/instrumentation/*veterinary
Capillaries/chemistry
Diabetic Ketoacidosis/diagnosis/therapy/*veterinary
Dog Diseases/*diagnosis/therapy
Dogs
Electrochemical Techniques/instrumentation/*veterinary
3-Hydroxybutyric Acid

Figure

• 

  Fig. 1 Spearman's correlation and Bland-Altam plots of (A) capillary hydroxybutyrate (3-HB) measured with a ketone sensor and venous hydroxybutyrate (3-HB) measured using the laboratory method (p < 0.0001, r = 0.80) and (B) venous 3-HB measured with a ketone sensor and venous 3-HB measured using a laboratory method (p < 0.0001, r = 0.94).

• 

  Fig. 2 Receiving operating characteristic curve of capillary 3-HB and urinary AcAc test assessed by comparing dogs with DK and DKA.

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