Korean J Lab Med.  2010 Dec;30(6):675-684. 10.3343/kjlm.2010.30.6.675.

Evaluation of Anti-dsDNA Antibody Tests: Crithidia luciliae Immunofluorescence Test, Immunoblot, Enzyme-linked Immunosorbent Assay, Chemiluminescence Immunoassay

• Yang JY
• Oh EJ
• Kim Y
• Park YJ

• KMID: 1781676
• DOI: http://doi.org/10.3343/kjlm.2010.30.6.675

Abstract

BACKGROUND
Anti-double stranded DNA antibody (anti-dsDNA) test is useful for the diagnosis and monitoring of systemic lupus erythematosus (SLE). Although several methods are available, none of them is completely satisfactory and differences among them have been reported. We evaluated the diagnostic performance of 6 commercial kits for anti-dsDNA detection.
METHODS
A total of 142 sera (SLE [N=74], other systemic rheumatic diseases [N=50], other diseases [N=18]) were tested by 6 different assay kits using different antigenic sources of DNA: Crithidia luciliae immunofluorescence test (CLIFT), salmon testes (immunoblot, IB), human (ELISA I), salmon testes with nucleosome linker (ELISA II), plasmid (ELISA III), and synthetic oligonucleotides (chemiluminescence immunoassay, CLIA).
RESULTS
With manufacturers' cut-off values, 6 test kits showed sensitivities of 55.4-91.9%. ELISA I had a greater sensitivity than the other five assays (P<0.001). The specificities of ELISA II, ELISA III, CLIA, and CLIFT were higher than those of ELISA I and IB (P<0.05). In ROC curve analysis, 3 ELISA kits and CLIA showed AUC values of 0.845-0.893, and revealed no significant differences among them (P>0.05). With cut-off values set at 95% of specificity, ELISA II had a higher sensitivity than ELISA III (63.5% vs. 41.9%, P<0.05). IB had poor concordance rates with other assays (42.0-65.0%). Pearson correlation coefficients among 4 quantitative assays were 0.667-0.798.
CONCLUSIONS
Six different assays showed various performances depending on the methods and cut-off values used. Except IB, the other five assays can be used for the detection of anti-dsDNA.

Keyword

Anti-dsDNA; Crithidia luciliae immunofluorescence test; ELISA; Chemiluminescence immunoassay; Immunoblot assay

MeSH Terms

Adult
Antibodies, Antinuclear/*analysis
Area Under Curve
Chemiluminescent Measurements/*methods
DNA/*immunology
Enzyme-Linked Immunosorbent Assay/*methods
Female
Fluorescent Antibody Technique/*methods
Humans
Immunoblotting/*methods
Lupus Erythematosus, Systemic/diagnosis
Male
Middle Aged
ROC Curve
Reagent Kits, Diagnostic
Sensitivity and Specificity