Korean J Lab Med.  2007 Dec;27(6):428-436. 10.3343/kjlm.2007.27.6.428.

Coproduction of qnrB and armA from Extended-Spectrum beta-lactamase-producing Klebsiella pneumoniae

• Kim MH
• Sung JY
• Park JW
• Kwon GC
• Koo SH

• KMID: 1781547
• DOI: http://doi.org/10.3343/kjlm.2007.27.6.428

Abstract

BACKGROUND: Multidrug-resistant Enterobacteriaceae is a worldwide problem. Although various resistance mechanisms have been recognized with increasing frequency, only a few cases of triple resistance of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae have been reported. This study was designed to evaluate the coexistence of qnr (qnrA, qnrB, and qnrS) and 16S rRNA methylase (armA, rmtA, rmtB, and rmtC) in ESBL-producing K. pneumoniae. METHODS: We tested 44 isolates of ESBL-producing K. pneumoniae at Chungnam National University Hospital from March to September 2006. Antimicrobial susceptibilities were tested by broth microdilution method, and transconjugation test was performed using E. coli J53 with azide resistance. Search for qnr (qnrA, qnrB, and qnrS) and 16S rRNA methylase (armA, rmtA, rmtB, and rmtC) genes was conducted by PCR amplification, and the genotypes were determined by direct nucleotide sequence analysis of the amplified products. Epidemiologic study was performed by Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR). RESULTS: All ESBL-positive strains produced qnrB; however, armA was detected in 68.2%. The coproduction rate of qnrB and armA in ESBL-producing K. pneumoniae was 68.2%. Two types (A and B) were dominant in ERIC-PCR results. CONCLUSIONS: K. pneumoniae producing qnrB, armA, and ESBL are spreading widely.

Keyword

Extended-spectrum beta-lactamase; qnrB; armA; Klebsiella pneumoniae

MeSH Terms

Bacterial Proteins/biosynthesis/*genetics
Disk Diffusion Antimicrobial Tests
Drug Resistance, Bacterial/genetics
Humans
Klebsiella Infections/microbiology
Klebsiella pneumoniae/drug effects/*genetics/isolation & purification
Methyltransferases/biosynthesis/*genetics
beta-Lactamases/biosynthesis/drug effects/*genetics

Figure

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  Fig. 1. Enterobacterial repetitive intergenic consensus-PCR patterns of genomic DNA from clinical isolates of K. pneumoniae harboring ESBLs. Lane M is 1kb DNA size marker. Among the five different banding patterns (A, B, C, D, E), A and B patterns are dominant. Thirty nine of 44 extended-spectrum β-lactamase -producing clinical isolates show A or B patterns.

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