J Korean Med Sci.  2009 Aug;24(4):547-554. 10.3346/jkms.2009.24.4.547.

Comparison of Cytokine Expression in Mesenchymal Stem Cells from Human Placenta, Cord Blood, and Bone Marrow

• Hwang JH
• Shim SS
• Seok OS
• Lee HY
• Woo SK
• Kim BH
• Song HR
• Lee JK
• Park YK

• KMID: 1779182
• DOI: http://doi.org/10.3346/jkms.2009.24.4.547

Abstract

Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation into lineages of mesenchymal tissues that are currently under investigation for a variety of therapeutic applications. The purpose of this study was to compare cytokine gene expression in MSCs from human placenta, cord blood (CB) and bone marrow (BM). The cytokine expression profiles of MSCs from BM, CB and placenta (amnion, decidua) were compared by proteome profiler array analysis. The cytokines that were expressed differently, in each type of MSC, were analyzed by real-time PCR. We evaluated 36 cytokines. Most types of MSCs had a common expression pattern including MIF (GIF, DER6), IL-8 (CXCL8), Serpin E1 (PAI-1), GROalpha(CXCL1), and IL-6. MCP-1, however, was expressed in both the MSCs from the BM and the amnion. sICAM-1 was expressed in both the amnion and decidua MSCs. SDF-1 was expressed only in the BM MSCs. Real-time PCR demonstrated the expression of the cytokines in each of the MSCs. The MSCs from bone marrow, placenta (amnion and decidua) and cord blood expressed the cytokines differently. These results suggest that cytokine induction and signal transduction are different in MSCs from different tissues.

Keyword

Mesenchymal Stem Cells; Placenta; Fetal Blood; Bone Marrow; Cytokines

MeSH Terms

Bone Marrow Cells/*cytology
Cytokines/genetics/*metabolism
Female
Fetal Blood/*cytology
Gene Expression Profiling
Humans
Mesenchymal Stem Cells/cytology/*metabolism
Placenta/*cytology
Pregnancy
Protein Array Analysis

Figure

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  Fig. 1 Appearance and growth of fibroblastoid cells or placental mesenchymal stem cells at passage 1 on day 11.

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  Fig. 2 Immunophenotypic results of passage 3 MSCs by FACS analysis. (A) MSC from amnion (B) MSC from decidua. Representative histogram (black line). The respective isotype control is shown as gray.

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  Fig. 3 Differentiation potential of MSCs obtained from the amnion and decidua. (A) Adipogenic differentiation of MSC shown by Oil red O staining of adipocytes (×200). (B) Osteogenic differentiation of MSC shown by Alizarin red S (×200). (A, amnion; D, decidua).

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  Fig. 4 (A) The cytokine expression in each of the MSCs using the proteome profiler. (B) Quantification of cytokine optical density. Measurements were obtained with the Image Pro Plus v 5.1 software (Media Cybernetics, Silver Spring, MD, U.S.A.). CB, cord blood; BM, bone marrow; AM, amnion; DE, decidua MSCs; MSC, mesenchymal stem cells.

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  Fig. 5 The quantitative expression of SDF-1, MCP-1 and sICAM-1 in each of the mesenchymal stem cells. The mRNA levels were quantified using TaqMan Real-Time PCR with an ABI 7700 system (Applied Biosystems). The GAPDH housekeeping gene product was used as an endogenous reference. (A) SDF-1, (B) MCP-1, (C) sICAM-1. *Statistically significant difference (P<0.05). SDF-1, stromal derived factor-1; MCP-1, monocyte chemotactic protein-1; sICAM-1, intracellular adhesion molecule.

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  Fig. 6 SDF-1, MCP-1 and sICAM-1 expression profile by Western blot analysis in amnion-derived MSC and decidua-derived MSC. AM MSC, amnion-derived MSC; DE MSC, decidua-derived MSC.

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