Yonsei Med J.  2009 Aug;50(4):493-504. 10.3349/ymj.2009.50.4.493.

Immunoglobulin VH Chain Gene Analysis of Peripheral Blood IgM-Producing B Cells in Patients with Kawasaki Disease

• Lee HH
• Shin JS
• Kim DS

• KMID: 1758607
• DOI: http://doi.org/10.3349/ymj.2009.50.4.493

Abstract

PURPOSE
Kawasaki disease is a systemic vasculitis, and its etiology and pathogenesis are still not clear. Our study was undertaken to investigate the characteristics of the activation of B cells in the peripheral blood of Kawasaki disease (KD) patients and evidence of stimulation by superantigens. MATERIALS AND METHODS: Blood samples were obtained from three patients (2 males, 1 female) with KD, who were admitted to our Hospital, Seoul, Korea. The mean age was 1.2 years. Distribution of B cells was studied in the acute and subacute phases of KD patients. From the RNA of B cells, we obtained complementary DNA (cDNA) and performed polymerase chain reaction (PCR). To determine the oligoclonal expansion of immunoglobulin M (IgM) VH family, we cloned and sequenced the PCR products from each group and analyzed DNA. RESULTS: In the peripheral blood of acute phase patients, T cells were significantly decreased (p < 0.05), whereas B cells were significantly increased (p < 0.05). When the first PCR was done on the B cell chains, VH1 to VH6 were all found to be expressed. The number of micro gene clones obtained from 3 patients was 312, and they belonged to VH3, VH4 and VH5 family. M99686 germ line was most frequently used and the next most frequently used, were X92224/J, L21967 and L21964. A similar order was seen in patients. Among the clones, 20 sets of clones showed the same base sequence and this was frequent between VH2 and VH5. There was one set, which showed almost the same base sequence between different patients, and the homology was 99.5%. Twenty sets of clones that had the same base sequence showed high similarity to the germ line (94 - 100%). Among these, the clones that utilized the M99686 germ line were 4 sets which were most frequent. The 3-dimensional structure of one of these clones showed typical beta, sheet structure of immunoglobulin chains. CONCLUSION: The IgM transcripts expressed by the B cells in the peripheral blood of KD patients in the acute phase of the disease clearly showed an oligoclonal expansion, suggesting that KD is caused not by stimulation of a superantigen, but rather by a conventional antigen.

Keyword

Kawasaki disease; B cell; oligoclonal expansion; superantigen; conventional antigen

MeSH Terms

B-Lymphocytes/*metabolism
Female
Humans
Immunoglobulin M/*metabolism
Immunoglobulin Variable Region/*genetics
Infant
Male
Mucocutaneous Lymph Node Syndrome/*genetics/*immunology
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA

Figure

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  Fig. 1 Amplification of V regions of Ig µ heavy chain genes (VH1 - VH6) by RT-PCR. M, marker; RT-PCR, reverse transcription polymerase chain reaction.

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  Fig. 2 The deduced amino acid sequences of VH germ line gene segments of 54 clones isolated from three different patients are shown in comparison with germ line M99686.

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  Fig. 3 The nucleotide sequences of CDR3 / FR4 and adjacent regions of VH germ line gene segments of 54 clones homologous to M99686 isolated from 3 different patients are shown in comparison with germ line M99686.

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  Fig. 4 Mutation analysis of 54 M99686 derived clones from the peripheral B cells of 3 different patients.

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  Fig. 5 The deduced amino acid sequences of VH germ line gene segments of 54 clones isolated from 3 different patients are shown in comparison with germ line X92224/J.

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  Fig. 6 The nucleotide sequences of CDR3 / FR4 and adjacent regions of VH germ line gene segments of 54 clones homologous to X92224/J isolated from 3 different patients are shown.

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  Fig. 7 Mutation analysis of 48 X92224/J derived clones from peripheral B cells of 3 different patients.

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  Fig. 8 Sequence alignment of 5-1-8 (patient #1) vs. 5-3-4 (patient #3) clones. The sequence of clones showed almost identical alignment.

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