Korean J Ophthalmol.  1990 Jun;4(1):1-4. 10.3341/kjo.1990.4.1.1.

Detection of specific proteins in the aqueous humor in primary open-angle glaucoma

• Lee IS
• Yu YS
• Kim DM
• Youn DH
• Kim JQ

• KMID: 1735052
• DOI: http://doi.org/10.3341/kjo.1990.4.1.1

Abstract

To elucidate the mechanism of increased intraocular pressure in primary open-angle glaucoma (POAG), the protein profiles of aqueous humor obtained from POAG patients were compared with those of cataract patients as a control group. Aqueous humor proteins were analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and detected by the ultrasensitive silver staining technique. In 79% of the samples taken from POAG patients, protein bands of 140,000 or 160,000 daltons were stained, but none were stained from cataract patients. The presence of these protein bands revealed statistically significant differences between the two groups. Protein bands of 140,000 or 160,000 daltons were evenly visible at all ages in POAG patients, and the positivity of bands had no correlation with sex or initial intraocular pressure level. It is possible that the ultrastructural changes of the aqueous outflow pathway in POAG may be related to the changes in the aqueous protein, presence of 140,000 or 160,000 daltons protein bands.

Keyword

aqueous humor; protein; primary open-angle glaucoma; sodium dodecylsvlfate-polyacrylamide gel electrophoresis; silver staining

MeSH Terms

Adult
Aged
Aqueous Humor/*metabolism
Cataract/metabolism
Electrophoresis, Polyacrylamide Gel
Eye Proteins/*metabolism
Female
Glaucoma, Open-Angle/*metabolism
Humans
Male
Middle Aged
Molecular Weight
Eye Proteins