J Korean Med Sci.  2000 Feb;15(1):88-92. 10.3346/jkms.2000.15.1.88.

Expression of cyclooxygenase-1 and -2 in rheumatoid arthritis synovium

• Lee YH
• Choi SJ
• Kim A
• Kim CH
• Ji JD
• Song GG

• KMID: 1731241
• DOI: http://doi.org/10.3346/jkms.2000.15.1.88

Abstract

The aim of this study was to investigate the expression and localization of cyclooxygenase-1 and -2 (COX-1 and COX-2) in synovial tissues from patients with rheumatoid arthritis (RA). Synovial tissues from 9 patients with RA and 5 patients with osteoarthritis (OA) were examined for COX-1 and COX-2 expressions by immunohistochemical staining using 2 polydonal COX-1 and COX-2 antibodies. In RA synovia, synovial lining cells showed intense immunostaining for COX-1, whereas slight to moderate staining was observed in inflammatory cells, stromal fibroblast-like cells and vascular endothelial cells. There was no significant difference in COX-1 expression between RA and OA synovia. The localization of COX-2 expression dearly differed from that of COX-1 expression, being most intense in inflammatory cells. However, there was no difference in COX-1 and COX-2 expressions between RA and OA synovial tissues. Our observations support that inflammatory mechanisms modulated by COX-1 and COX-2 in chronic RA synovium might be similar to those in chronic OA synovium.

Keyword

Arthritis, Rheumatoid; Prostaglandin-Endoperoxide Synthase; Synovial Membrane

MeSH Terms

Adult
Aged
Arthritis, Rheumatoid/pathology
Arthritis, Rheumatoid/enzymology*
Cell Division
Female
Fibrin/metabolism
Human
Isoenzymes/metabolism
Isoenzymes/biosynthesis*
Male
Middle Age
Neutrophil Infiltration
Osteoarthritis/enzymology
Prostaglandin-Endoperoxide Synthase/metabolism
Prostaglandin-Endoperoxide Synthase/biosynthesis*
Stromal Cells/pathology
Stromal Cells/enzymology
Synovial Membrane/pathology
Synovial Membrane/enzymology*