J Korean Med Sci.  2000 Aug;15(4):387-392. 10.3346/jkms.2000.15.4.387.

A comparison study on allergen components between Korean (Arachis fastigiata Shinpung) and American peanut (Arachis hypogaea Runner)

• Park CW
• Kim GI
• Lee CH

• KMID: 1730633
• DOI: http://doi.org/10.3346/jkms.2000.15.4.387

Abstract

The prevalence of peanut allergy in Korea is lower than in America. Peanut extract allergens between the two countries have not been standardized. This study was performed to compare the allergenicity of raw Korean and American peanuts with that of roasted peanuts. We prepared the peanut extracts in Korean raw (KP) and roasted peanuts (KRP), and also in American raw (AP) and roasted (ARP) peanuts. We compared the peanut extract allergens of KP, KRP, AP and ARP in vitro with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting, T-cell proliferation assay and skin prick test with sera from peanut-allergic patients. SDS-PAGE and Western blotting demonstrated four allergenic extracts, numerous bands that displayed a high prevalence of IgE binding. IgE-binding bands were at 64, 36 and 17 kDa. Western blot inhibition revealed that either KP or AP could almost completely inhibit the reactivity of the other extract. There were no differences between T-cell proliferation assay and skin prick test. In conclusion, this investigation showed no different allergic components in both raw and roast extracts of Korean and American peanuts.

Keyword

Peanut Allergy; Peanut Allergens; Food Hypersensitivity

MeSH Terms

Allergens/immunology
Allergens/analysis+ACo-
Allergens/adverse effects
Blotting, Western
Cells, Cultured
Comparative Study
Electrophoresis, Polyacrylamide Gel
Food Hypersensitivity/immunology
Heat
Human
Hybridization
Korea
Lymphocyte Transformation
North America
Peanuts/immunology
Peanuts/classification
Peanuts/chemistry+ACo-
Plant Extracts/immunology
Plant Proteins/immunology
Plant Proteins/analysis+ACo-
Plant Proteins/adverse effects
Skin Tests
Species Specificity
T-Lymphocytes/immunology
T-Lymphocytes/drug effects