J Vet Sci.  2009 Dec;10(4):299-304. 10.4142/jvs.2009.10.4.299.

Infrared spectroscopy characterization of normal and lung cancer cells originated from epithelium

• Lee SY
• Yoon KA
• Jang SH
• Ganbold EO
• Uuriintuya D
• Shin SM
• Ryu PD
• Joo SW

• KMID: 1726901
• DOI: http://doi.org/10.4142/jvs.2009.10.4.299

Abstract

The vibrational spectral differences of normal and lung cancer cells were studied for the development of effective cancer cell screening by means of attenuated total reflection infrared spectroscopy. The phosphate monoester symmetric stretching nus(PO3(2-)) band intensity at ~970 cm-1 and the phosphodiester symmetric stretching nus(PO2-) band intensity at ~1,085 cm-1 in nucleic acids and phospholipids appeared to be significantly strengthened in lung cancer cells with respect to the other vibrational bands compared to normal cells. This finding suggests that more extensive phosphorylation occur in cancer cells. These results demonstrate that lung cancer cells may be prescreened using infrared spectroscopy tools.

Keyword

cancer cell screening; cell monitoring; infrared spectroscopy; lung cancers; phosphorylation

MeSH Terms

*Carcinoma
Cell Line, Tumor
Epithelial Cells/*physiology
Humans
*Lung Neoplasms
Respiratory Mucosa/*cytology
*Spectrophotometry, Infrared

Figure

• 

  Fig. 1 Infrared spectra of (a) normal human bronchial epithelial (NHBE) and (b) NCI-H358 and (c) NCI-H460 lung cancer cells. The spectra for each sample were read twice to check reproducibility. The intensities were normalized with respect to that of the amide I band at ~1,650 cm-1.

• 

  Fig. 2 Relative band intensities for NCI-H358 and NCI-H460 lung cancer cells and normal NHBE-P5 cells for (a) νs(PO32-), (b) νs(PO2-), (c) νas(PO2-), (d) δas(CH3), (e) δs(CH3), (f) amide II, and (g) amide I bands. The intensities were normalized for the amide I bands at ~1,650 cm-1.

• 

  Fig. 3 Spectral curve fitting of (a) NHBE and (b) NCI-H358 and (c) NCI-H460 lung cancer cells in the wavenumber region of 900~1,290 cm-1. Each sample was tested twice to check the spectral reproducibility. The y-axis scale is the absorbance in arbitrary units.

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