Korean J Parasitol.  2005 Sep;43(3):119-122. 10.3347/kjp.2005.43.3.119.

A new method for concentration of proteins in the calcareous corpuscles separated from the spargana of Spirometra erinacei

Affiliations
  • 1Department of Parasitology, College of Medicine, Inha University, Incheon 400-712, Korea.
  • 2Department of Parasitology and Tropical Medicine, Seoul National University, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea. cjy@snu.ac.kr

Abstract

Calcareous corpuscles are a characteristic structure found in larval and adult stage cestodes. These corpuscles are known to contain several protein components and to possess protein-binding activity. However, the proteins bound to calcareous corpuscles in situ have not been studied. The present study was undertaken to identify the proteins on calcareous corpuscles. Calcareous corpuscles were purified from the plerocercoids (= spargana) of Spirometra erinacei, and serially dissolved using 0.1 M sulfamic acid solution. Collected supernatants were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver staining. The results showed that only the fraction remaining after the 19th dissolved fraction contained proteins. A total of 20 protein molecules were detected in gel, with major bands at 56, 53, 46, 40, 35, 29, 28, 24.5, 21, 19, 16, 13, 10 and 8 kDa. In particular, the proteins corresponding to the 21 and 16 kDa bands were most abundant. Our results demonstrated for the first time the protein contents of the calcareous corpuscles of spargana. Further studies on the functions of these proteins are required.

Keyword

Spirometra erinacei; sparganum; plerocercoid; calcareous corpuscle; binding proteins

MeSH Terms

Animals
Centrifugation
Electrophoresis, Polyacrylamide Gel
Helminth Proteins/analysis/*metabolism
Molecular Weight
Protein Binding
Research Support, Non-U.S. Gov't
Silver Staining
Sparganum/isolation & purification/*metabolism
Spirometra/*metabolism
Sulfonic Acids
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