Korean J Parasitol.  2000 Sep;38(3):145-150. 10.3347/kjp.2000.38.3.145.

IgG antibody responses in early experimental sparganosis and IgG subclass responses in human sparganosis

• Chung YB
• Kong Y
• Yang HJ
• Cho SY

• KMID: 1720316
• DOI: http://doi.org/10.3347/kjp.2000.38.3.145

Abstract

Antigenic components in the crude extracts of Spirometra mansoni plerocercoid were analyzed in early experimental infections and in IgG subclass observed in clinical sparganosis. By IgG immunoblot, sera obtained serially from experimental mice, fed 5 spargana each, were reacted with the crude extracts. Protein bands at 36-26 kDa and 103 kDa showed positive reactions since two weeks after infection. In a differential immunoblot, in which a monospecific antibody against sparganum chymase at 36 kDa was pre-treated, the reactions at 36-26 kDa disappeared, indicating that the sparganum chymase and its degradation products invoked IgG antibody reactions. When 69 patients sera of human sparganosis were examined for their IgG subclass responses, IgG4 levels showed the highest reaction which was followed by IgG1. The IgG4 antibody also reacted mainly with 36-31 kDa protease. These results indicate that 36 kDa chymase of S. mansoni plerocercoid is the main antigenic component inducing IgG antibody response in early stage of experimental sparganosis and for specific IgG subclass reactions in human sparganosis.

MeSH Terms

Animal
Antibodies, Helminth/blood*
Antigens, Helminth/immunology
Human
IgG/classification
IgG/blood*
Immunoblotting
Mice
Mice, Inbred ICR
Molecular Weight
Serine Endopeptidases/immunology
Sparganosis/immunology*
Sparganum/immunology
Sparganum/enzymology