Korean J Parasitol.  2011 Mar;49(1):45-49. 10.3347/kjp.2011.49.1.45.

Influencing Factors for Cure of Clonorchiasis by Praziquantel Therapy: Infection Burden and CYP3A5 Gene Polymorphism

• Kim CH
• Lee JK
• Chung BS
• Li S
• Choi MH
• Hong ST

• KMID: 1715632
• DOI: http://doi.org/10.3347/kjp.2011.49.1.45

Abstract

Chemotherapy of clonorchiasis with praziquantel (PZQ) is effective but about 15% of treated cases have been reported uncured. The present study investigated correlation of single nucleotide polymorphisms (SNPs) of the cytochrome P450 gene, CYP3A5 and cure of clonorchiasis. A total of 346 egg passing residents were subjected and treated by 3 doses of 25 mg/kg PZQ. Reexamination recognized 33 (9.5%) uncured and 313 cured. Numbers of eggs per gram of feces (EPGs) before treatment were significantly lower in the cured group than in the uncured group (2,011.2+/-3,600.0 vs 4,998.5+/-7,012.0, P<0.001). DNAs of the subjects were screened for SNPs at 7 locations of CYP3A5 using PCR. In the uncured group, the SNP frequencies at g.-20555G>A and g.27526C>T of CYP3A5 were 15.2% and 9.1% while those were 3.8% and 1.0%, respectively, in the cured group. The cure rate was significantly lower in the cases with SNP at g.27526C>T and EPGs> or =1,000. In conclusion, EPGs and SNPs of CYP3A5 are factors which influence cure of clonorchiasis by PZQ therapy. It is strongly suggested to recommend 2-day medication for individuals with high EPGs> or =1,000.

Keyword

Clonorchis sinensis; clonorchiasis; praziquantel; cyochrome P450; CYP3A5; single nucleotide polymorphism (SNP); EPG

MeSH Terms

Adolescent
Adult
Aged
Aged, 80 and over
Animals
Child
Clonorchiasis/*drug therapy/*genetics/parasitology
Clonorchis sinensis/isolation & purification/physiology
Cytochrome P-450 CYP3A/*genetics
Feces/parasitology
Female
Humans
Male
Middle Aged
*Polymorphism, Single Nucleotide
Praziquantel/*therapeutic use
Treatment Outcome
Young Adult