Korean J Parasitol.  1991 Dec;29(4):397-401. 10.3347/kjp.1991.29.4.397.

Serum IgE levels in rats infected with Paragonimus westermani

• Shin MH
• Ryu JS
• Min DY

• KMID: 1715544
• DOI: http://doi.org/10.3347/kjp.1991.29.4.397

Abstract

Paragonimus westermani is a common fluke in Korea. The present study aimed to determine serum total IgE and specific IgG levels in experimental paragonimiasis of rats. Each Wistar rat was inoculated orally with 20-25 metacercariae of P. westermani from Cambaroides similis. Before and after infection (1, 2, 3, 4, 6, 8 weeks) of P. westermani, the blood was collected from the retro-orbital venous plexus of rats and kept serum at -70 degrees C. Serum total IgE and specific IgG levels were determined by the capture and conventional enzyme-linked immunosorbent assay, respectively. The results were as follows; 1. Serum IgE values were increased to 0.18 +/- 0.042 at 2 weeks, 0.28 +/- 0.151 at 4 weeks and 0.43 +/- 0.055 at 8 weeks after infection. The absorbances of non-infected rats ranged 0.07 +/- 0.021-0.12 +/- 0.025. 2. Specific IgG values were slightly increased at 3 weeks (0.20 +/- 0.032) and gradually increased up to 8 weeks (0.31 +/- 0.067) after infection. The absorbances of non-infected rats ranged 0.11 +/- 0.035-0.18 +/- 0.019. The present results suggested that P. westermani could elevate serum IgE and specific IgG antibodies in Wistar rats which were not a good definitive host.

MeSH Terms

English-Abstract
IgG-metabolism
Rats-
Rats,-Inbred-Strains
*Antibodies,-Helminth-metabolism
*IgE-metabolism
*Paragonimiasis-immunology
*Paragonimus-immunology
Antibodies,-Helminth
IgG
IgE