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Purification of antigenic protein of sparganum by immunoaffinity chromatography using a monoclonal antibody

Cho SY, Kang SY, Kong Y

The quality improvement of antigen (crude saline extract) of Spirometra mansoni pleroceroid (sparganum) was investigated by protein purification. The crude extract was fractionated by gel filtration through Sephacryl S-300 Superfine....
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Immunohistochemical observation on the antigens inducing IgG and IgM antibodies against sparganum

Kim CH, Choi WS

Localization and characterization of the antigenic components of sparganum which induced IgG and IgM antibodies in the host were studied by immunohistochemical techniques and SDS-PAGE and Western blotting. The antigen...
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Applicability of ABC-ELISA and protein A-ELISA in serological diagnosis of cysticercosis

Lee JH, Kong Y, Ryu JY, Cho SY

Specific antibody test in serum and cerebrospinal fluid (CSF) is still the main mode of serological diagnosis of cysticercosis. Of different techniques of antibody test, enzyme-linked immunosorbent assay (micro-ELISA) has...
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IgA response in mice infected with Neodiplostomum seoulensis

Huh S, Lee SU, Won MH, Jeong YG, Kwon YH, Choi CS

To observe the production of IgA in Balb/c mice with neodiplostomiasis, 20 mice were infected with each 200 metacercariae of Neodiplostomum seoulenis. Sera and the duodenums were obtained 3, 7,...
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Immune reactions between excretory-secretory antigens and specific antibodies of Clonorchis sinensis before and after praziquantel treatment in experimentally infected rabbits

Kim SI

This study was designed to evaluate the humoral immune reactions in clonorchiasis before and after praziquantel treatment. Rabbits were infected with 150 or 450 metacercariae, treated on 4 and 8...
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Serodiagnosis of cysticercosis by ELISA-inhibition test using monoclonal antibodies

Yong TS, Yeo IS, Seo JH, Chang JK, Lee JS, Kim TS, Jeong GH

Monoclonal antibodies (Mabs) were produced against crude scolex extract of T. solium metacestodes, and applied to ELISA-inhibition test for improving the specificity of serodiagnosis of human cysticercosis. Four hybridomas...
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Serodiagnosis of human paragonimiasis by ELISA-inhibition test using monoclonal antibodies

Yong TS, Seo JH, Yeo IS

ELISA-inhibition test using Paragonimus westermani specific monoclonal antibody (Mab) was investigated to improve the diagnostic specificity of paragonimiasis. By cell fusion, one hybridoma clone secreting anti-P. westermani specific Mab was...
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Cross-reacting and specific antigenic components in cystic fluid from metacestodes of Echinococcus granulosus and Taenia solium

Kong Y, Kang SY, Cho SY, Min DY

Sera from confirmed patients of 5 hydatidosis, 67 neurocysticercosis and 89 other parasitic diseases were tested for specific antibody (IgG) levels by ELISA to cystic fluid antigens from metacestodes of...
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Serum IgE levels in rats infected with Paragonimus westermani

Shin MH, Ryu JS, Min DY

Paragonimus westermani is a common fluke in Korea. The present study aimed to determine serum total IgE and specific IgG levels in experimental paragonimiasis of rats. Each Wistar rat was...
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Immunohistochemical study on the antigenicity of each organ structure of Clonorchis sinensis

Kim J, Chai JY, Kho WG, Cho KH, Lee SH

An immunohistochemical study was performed to demonstrate comparative antigenicity of each body structure of the liver fluke, Clonorchis sinensis, such as the digestive tract, reproductive organs, excretory system, tegument and...
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Changes of IgE production, splenic helper and suppressor T lymphocytes in mice infected with Paragonimus westermani

Min DY, Ryu JS, Shin MH

Effects of Paragonims westermani infection were observed in mice on the change of serum IgE level, the number of peripheral eosinophils and the distribution of Thy 1.2+ (CD3), L3T4+ (CD4),...
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Changes of IgM and IgG antibody levels in experimental rabbit anisakiasis as observed by ELISA and SDS-PAGE/immunoblot

Yang HJ, Cho YJ, Paik YH

Antibody changes in experimental anisakiasis were observed in 10 rabbits which were infected each with 10 Anisakis simplex larvae. The sera were collected before and on the 6th to the...
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Antibody changes in paragonimiasis patients after praziquantel treatment as observed by ELISA and immunoblot

Cho SY, Kim SI, Kang SY, Kong Y, Han SK, Shim YS, Han YC

To observe antibody changes after praziquantel treatment in paragonimiasis, a total of 46 serum samples from 13 serologically diagnosed patients was collected for 4-28 months. The specific antibody (IgG) levels...
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The fate of spargana inoculated into the cat brain and sequential changes of anti-sparganum IgG antibody levels in the cerebrospinal fluid

Wang KC, Huh S, Hong ST, Chai JY, Choi KS, Lee SH

To establish an animal model of intracranial sparganosis, the fate and behavior of the experimentally inoculated spargana were observed. A total of 102 scolices of spargana were injected into 22...
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The effects of antibodies and complement in macrophage-mediated cytotoxicity on metacercariae of the lung fluke, Paragonimus westermani

Min DY, Ahn MH, Kim KM, Leem MH, Park SY

Paragonimus westermani is a tissue migrating parasite in the early stage until arriving at lung, and most of the parasites spend their life spans there. Considerable immune responses including activation...
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Immunohistochemical study on the antigenicity of body compartments of Paragonimus westermani

Lee SH, Sung SH, Chai JY

Production of circulating specific antibodies to the lung fluke (Paragonimus westermani) by its host is well known and used in various kinds of immunodiagnostic methods. However, it has not been...
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The changes of histopathology and serum anti-sparganum IgG in experimental sparganosis of mice

Hong ST, Kim KJ, Huh S, Lee YS, Chai JY, Lee SH, Lee YS

The present study is intended to observe the chronologic changes of experimental sparganosis by histopathological observation and detection of circulating anti-sparganum IgG antibody using ELISA. Each of 25 mice was...
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