Korean J Parasitol.  1989 Mar;27(1):15-21. 10.3347/kjp.1989.27.1.15.

Antibody changes in paragonimiasis patients after praziquantel treatment as observed by ELISA and immunoblot

Affiliations
  • 1Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
  • 2Department of Internal Medicine, Seoul National University Hospital, Seoul 110-744, Korea.

Abstract

To observe antibody changes after praziquantel treatment in paragonimiasis, a total of 46 serum samples from 13 serologically diagnosed patients was collected for 4-28 months. The specific antibody (IgG) levels were measured by enzyme-linked immunosorbent assay (ELISA). All but one patient who needed retreatment became symptom-free within a week. Antibody levels were dropped near to or below a cut-off absorbance (abs.) of 0.25 in varying intervals from 4 to 18 months. Of 9 patients who were retested within 3 months, 5 revealed temporary elevation of antibody level. After the elevation, the levels began to decline slowly to negative ranges. If treated earlier after symptoms developed, the temporary elevation did not occur and intervals to negative conversion were shorter. By sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)/immunoblot, antigen-antibody reactions in individual patient faded gradually without significant changes in reacting antigen bands.


MeSH Terms

Electrophoresis,-Polyacrylamide-Gel
Enzyme-Linked-Immunosorbent-Assay
Immunoblotting-
Paragonimiasis-drug-therapy
Paragonimiasis-immunology
*Antibodies,-Helminth-analysis
*IgG-analysis
*Paragonimiasis-diagnosis
*Paragonimus-immunology
*Praziquantel-therapeutic-use
Antibodies,-Helminth
Praziquantel
Full Text Links
  • KJP
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr