Korean J Parasitol.  1995 Sep;33(3):173-178. 10.3347/kjp.1995.33.3.173.

Effects of gamma-irradiation on intracellular proliferation of Toxoplasma gondii RH tachyzoites

• Kook J
• Oh SH
• Yun CK
• Chai JY

• KMID: 1715390
• DOI: http://doi.org/10.3347/kjp.1995.33.3.173

Abstract

A quantitative assay was performed on the effects of gamma-irradiation (30-300 Gy) on intracellular proliferation of Toxoplasma gondii RH tachyzoites in human leukemic HL-60 cells and murine peritoneal macrophages by means of 3H-uracil uptake assay. Infected non- irradiation group (NI) and uninfected group (incubating only host cells) were prepared. The 3H-uracil uptake by tachyzoites of NI group 12-24 hrs after infection was 2,190-4,787 counts per minute for macrophages and 2,967-8,254 for HL-60 cells, whereas the irradiated tachyzoites revealed only 381-703 (100 Gy) and 218-408 (300 Gy) for macrophages, and 1,911-2,618 (30 Gy), 1,253-1,384 (70 Gy), 1,013-1,090 (100 Gy), and 483-588 (300 Gy) for HL-60 cells. The proliferation inhibition rate was similar in macrophages and HL-60 cells, for example, 89-94% and 80-94% respectively by 300 Gy, 12-24 hrs after infection. It is concluded that RH tachyzoites of T. gondii are severely affected by gamma-irradiation in their capability of intracellular proliferation.

MeSH Terms

Animal
Cell Division/RADIATION EFFECTS
Cells, Cultured
Gamma Rays
Human
HL-60 Cells/PARASITOLOGY
Macrophages/PARASITOLOGY
Mice
Mice, Inbred BALB C
Mice, Inbred ICR
Radiation Dosage
Support, Non-U.S. Gov't
Toxoplasma/*RADIATION EFFECTS/*CYTOLOGY