Korean J Parasitol.  2009 Jun;47(2):117-124. 10.3347/kjp.2009.47.2.117.

IL-4 Independent Nuclear Translocalization of STAT6 in HeLa Cells by Entry of Toxoplasma gondii

• Ahn HJ
• Kim JY
• Nam HW

• KMID: 1720620
• DOI: http://doi.org/10.3347/kjp.2009.47.2.117

Abstract

Toxoplasma gondii provokes rapid and sustained nuclear translocation of the signal transducer and activator of transcription 6 (STAT6) in HeLa cells. We observed activation of STAT6 as early as 2 hr after infection with T. gondii by the nuclear translocation of fluorescence expressed from exogenously transfected pDsRed2-STAT6 plasmid and by the detection of phosphotyrosine-STAT6 in Western blot. STAT6 activation occurred only by infection with live tachyzoites but not by co-culture with killed tachyzoites or soluble T. gondii extracts. STAT6 phosphorylation was inhibited by small interfering RNA of STAT6 (siSTAT6). In view of the fact that STAT6 is a central mediator of IL-4 induced gene expression, activation of STAT6 by T. gondii infection resembles that infected host cells has been stimulated by IL-4 treatment. STAT1 was affected to increase the transcription and expression by the treatment of siSTAT6. STAT6 activation was not affected by any excess SOCS's whereas that with IL-4 was inhibited by SOCS-1 and SOCS-3. T. gondii infection induced Eotaxin-3 gene expression which was reduced by IFN-gamma. These results demonstrate that T. gondii exploits host STAT6 to take away various harmful reactions by IFN-gamma. This shows, for the first time, IL-4-like action by T. gondii infection modulates microbicidal action by IFN-gamma in infected cells.

Keyword

Toxoplasma gondii; entry; STAT6 phosphorylation; nuclear translocation; STAT1; IL-4; IFN-gamma

MeSH Terms

Active Transport, Cell Nucleus
Animals
Chemokines, CC/biosynthesis
Hela Cells
Humans
Interleukin-4/*immunology
Mice
Mice, Inbred BALB C
STAT6 Transcription Factor/*immunology/*metabolism
Toxoplasma/*immunology