Ann Lab Med.  2013 Jul;33(4):304-307. 10.3343/alm.2013.33.4.304.

The Utility of the Multiplex Reverse Transcriptase-Polymerase Chain Reaction Assay in the Detection of Hematologic Malignancies

• Kim MJ
• Cho SY
• Lee WI
• Park TS
• Lee HJ

• KMID: 1707242
• DOI: http://doi.org/10.3343/alm.2013.33.4.304

Abstract

No abstract available.

MeSH Terms

Adolescent
Aged
Child
Child, Preschool
Core Binding Factor Alpha 2 Subunit/analysis/genetics
Cytogenetic Analysis
Female
Fusion Proteins, bcr-abl/analysis/genetics
Hematologic Neoplasms/*diagnosis/genetics
Humans
Infant
Leukemia, Myeloid, Acute/diagnosis/genetics
Male
Middle Aged
*Multiplex Polymerase Chain Reaction
Oncogene Proteins, Fusion/analysis/genetics
Precursor Cell Lymphoblastic Leukemia-Lymphoma/diagnosis/genetics
*Reverse Transcriptase Polymerase Chain Reaction
Core Binding Factor Alpha 2 Subunit
Fusion Proteins, bcr-abl
Oncogene Proteins, Fusion

Reference

1. Swerdlow SH, Campo E, editors. WHO classification of tumours of haematopoietic and lymphoid tissues. 4th ed. Lyon: IARC Press;2008.

2. King RL, Naghashpour M, Watt CD, Morrissette JJ, Bagg A. A comparative analysis of molecular genetic and conventional cytogenetic detection of diagnostically important translocations in more than 400 cases of acute leukemia, highlighting the frequency of false-negative conventional cytogenetics. Am J Clin Pathol. 2011; 135:921–928. PMID: 21571965.

3. Lim G, Choi JR, Kim MJ, Kim SY, Lee HJ, Suh JT, et al. Detection of t(3;5) and NPM1/MLF1 rearrangement in an elderly patient with acute myeloid leukemia: clinical and laboratory study with review of the literature. Cancer Genet Cytogenet. 2010; 199:101–109. PMID: 20471513.

4. Kim MJ, Yoon HJ, Park TS. The e8a2 fusion transcript in B lymphoblastic leukemia with BCR-ABL1 rearrangement. Korean J Hematol. 2012; 47:161. PMID: 23071469.

5. Mitelman F, Mertens F, Johansson B. Prevalence estimates of recurrent balanced cytogenetic aberrations and gene fusions in unselected patients with neoplastic disorders. Genes Chromosomes Cancer. 2005; 43:350–366. PMID: 15880352.

6. Jamil A, Theil KS, Kahwash S, Ruymann FB, Klopfenstein KJ. TEL/AML-1 fusion gene. its frequency and prognostic significance in childhood acute lymphoblastic leukemia. Cancer Genet Cytogenet. 2000; 122:73–78. PMID: 11106814.

7. Kim SR, Kim HJ, Kim SH. Clinical utility of fluorescence in-situ hybridization profile test in detecting genetic aberrations in acute leukemia. Korean J Lab Med. 2009; 29:371–378. PMID: 19893343.

8. Huret JL. t(11;19)(q23;p13.1). Atlas Genet Cytogenet Oncol Haematol. 1997; 1:102–103. http://AtlasGeneticsOncology.org/Anomalies/t1119ELL.html.

9. Park TS, Lee SG, Song J, Lee KA, Kim J, Choi JR, et al. CASP8AP2 is a novel partner gene of MLL rearrangement with t(6;11) (q15;q23) in acute myeloid leukemia. Cancer Genet Cytogenet. 2009; 195:94–95. PMID: 19837277.

10. Kim MJ, Choi JR, Suh JT, Lee HJ, Lee WI, Park TS. Diagnostic standardization of leukemia fusion gene detection system using multiplex reverse transcriptase-polymerase chain reaction in Korea. J Korean Med Sci. 2011; 26:1399–1400. PMID: 22022200.

11. Yang JJ, Marschalek R, Meyer C, Park TS. Diagnostic usefulness of genomic breakpoint analysis of various gene rearrangements in acute leukemias: a perspective of long distance- or long distance inverse-PCR-based approaches. Ann Lab Med. 2012; 32:316–318. PMID: 22779077.

12. Shaffer LG, Slovak ML, editors. ISCN (2009): an international system for human cytogenetic nomenclature. Basel: S. Karger;2009.